Taibai M Different Active Site On Blood Coagulation And Myocardial Ischemia

Objective: With the direction of TCM theory and modern medical scientific theory , combine the physical and chemical properties containing composition of Notholirion bulbuliferun , study the experiment through the drug effect, set up Notholirion bulbuliferun determining the nature and quantitative differentiation method very much, confirm the material base of the efficiency, lay a foundation for drawing the craft and quality level . Study through the pharmacology experiment , external thrombus test, blood platelet gather test, blood rheology experiment research , cardiac muscle ischemic experiment study , investigate Notholirion bulbuliferun function and mechanism to myocardiac ischemia, offer the theoretical foundation for the fact that clinical application and new medicine are researched and developed. Methods Make up copy go on , draw , separate , carry on ration and determine the nature differentiation with thin layer chromatogram and high-efficient liquid phase chromatograph each in the Notholirion bulbuliferun too, pharmacology is studied and screened to the effective position in the Notholirion bulbuliferun very much. Pharmacology is studied: 1 .Through come out mouse external thrombus test , adopt different Epinephrine Notholirion bulbuliferun bath lead to the fact the blood silt model, determine thrombus length , wet weight, investigate the impact on indexes such as myocardial ,etc. of the thrombosis factor. 2.Assemble test through blood platelet, adopt collagen for urge , congeal pharmaceutical , determine blood platelet person who gather and slope, investigate the impact on indexes such as coronary heart disease ,etc. of the blood platelet factor. 3.Study through big mouse blood rheology experiment, adopt different Epinephrine ice bath lead to the fact the blood silt model, determine the whole blood viscosity , blood plasma viscosity, erythrocyte pigeonhole , accumulate , investigate Notholirion bulbuliferun effective position impact on indexes such as rheology of big mouse's blood. 4. Through anaesthetize dog cardiac muscle ischemic experiment study , adopt ligature anaesthetize dog lower , prop up , cause acute myocardial ischemia pathologic model in front of the left coronary artery, adopt the electric picture of membrane outside the heart (EECG ) , creatine kinase (CK ) , enzyme lactic dehydrogenase (LDH ), myocardial infarction range,etc. index observe...