Sugar-resistant Granules Interfere With The Kkay Mice Insulin Resistance Mechanism

In this study, we use Chinese herbs Tang Nai Kang to intervent IR c-onducting of KKAy diabetic mice, discuss the possible mechanism and w-ant to find new targets and new drugs to prevent type 2 diabetes.This paper includes two parts: review and experimental study.Review includes: " TCM research Overview of insulin resistance in ty-pe 2 diabetes " and " modern medicine research Overview of insulin resi-stance in type 2 diabetes".All recent relevant literature of type 2 diabetes IR research are docu-mented, we summarise pathogenesis, therapeutic medication, research meth-ods of type 2 diabetes IR in modern medicine and TCM . We discuss fu-rtherly the pathogenesis of type 2 diabetes IR in TCM.Study : Objective:To observe the effects of Tang Nai Kang in KKAy diabetic mice.Methods : KKAy diabetic mice were fed with high calorie di-et divided into control group, Tang Nai Kang high dose group, Tang Nai Kang low dose group and positive drug pioglitazone control group. C57bl/6J mice as a negative control. After 8 weeks,glucose and lipid metabolism, the insulin sensitivity, pathomorphology of pancreas and kidney ,tumor ne-crosis factor (TNF) -αlevels, free fatty acid (FFA) levels and renal expre-ssion of ICAM-1 were observed.Results : After eight weeks treatment withTang Nai Kang, FPG, Fins levels, ISI and TNF-αwere significantly chang-ed. Blood Fat and FFA were decreasing, the reason may be short on time, the mechanism yet to be further explored. HE pancreastic tissue staining s-howed that Tang Nai Kang can effectively reduce the degree of B cell de-pletion particles and vacuolation, keep the cell shape.Kidney HE staining r-evealed: After 8 weeks'treatment of Tang Nai Kang, the tubular membranenodular-glass-like material deposition, tubularepithelial cells in vacuolar de-generation, inflammatory cell infiltration in KKAy diabetic mice have varyi-ng degrees of improvement. ICAM-1 expression in renal:Tang Nai Kang ca-n obviously reduce the expression of ICAM-1 in KKAy diabetic mice's ren-al.Conclusion : The results show (1) Tang Nai Kang can effectively contr-ol insulin resistance in KKAy mice and delay the failure of B cells.The m-echanism may regulate the level of TNF-αand FFA. (2) Tang Nai Kang c-an prevent the occurrence and development of vascular lesions,protect renal function,and delay the development of DN in KKAy diabetic mice.But ho-w can Tang Nai Kang reduce the expression of ICAM-1 remains to be further studied.