| Aminoglycoside Antibiotics(AGS) is one of the most common antibiotics, they are used widely in clinical medicine. But all of these antibiotics have some bad influence for health, especially for our kidney and ears. Recently, some new AGS are synthesized, such as Etimicin Sulfate (ETIS) and Netilmicin Sulfate (NETS) , they have broad anti-bacterial spectrum and less poisonous than before. Toxicity of these drugs is related with their concentration in blood. In order to use these AGS scientifically and safely, it is very necessary and significative to find a new method to determine these antibiotics, especially for trace detection.Resonance Rayleigh Scattering Spectra is a new analytical technology, it is developed from 1990, it is paid attention by people for its high sensitivity and facility. Ion-association complexes which are formed by electrostatic gravitation, hydro force, charge change between small molecules as well as big biologic molecules' gather can produce strong RRS. It has been used to investigate nucleic acid, protein, amylose etc. , it can also determinate trace inorganic ion, organic matter and medicaments. RRS is so sensitive that it has fascinating foreground at life sciences, environment sciences and nano-meter materials, but there aren't any reports to detect NETS and ETIS with RRS .In this dissertation, the recent references in Aminoglycoside Antibiotics and Resonance Rayleigh Scattering Spectra were reviewed. Resonance Rayleigh Scattering Spectra (RRS) of interaction of Aminoglycoside Antibiotics with acid bisazo dye trypan blue (TB), acid bisazo dye evans blue (EB) and their analytical applications are investigated. The detail is given below.(1) In weak acid medium , some aminoglycoside antibiotics , such as Etimicin Sulfate (ETIS) and Netilmicin Sulfate (NETS) or acid bisazo dye trypan blue (TB) alone can only produce very weak Resonance Rayleigh Scattering (RRS) signals. However , when one of the antibiotics and TB react with each other to form the ion association complexes , the RRS intensity can be enhanced greatly and a new RRS spectrum and a significant enhancement of the RRS intensity in the wavelength range from 350 to 400 nm and 680-730nm can be observed. The maximum scattering peak is at 717 nm and there is a linear relationship between the RRS intensity and the antibiotics concentration in the range of 1.0-7.0μg·mL-1. An RRS method for the determination of aminoglycoside antibiotics at trace amounts of levels has been developed. This method has good selectivity and has been successfully applied to the quick determination of antibiotics not only for injections and ear drips , but also for clinic serum samples as well.(2) In about pH 4.2 weakly acidic medium, acid bisazo dye evans blue (EB) can react with ETIS and NETS. As a result, the spectra of absorption and fluorescence were changed a little, but the RRS was enhanced greatly, and a new RRS spectrum appeared. The maximum RRS signals of ion-association complexes were all located at about 713 nm .There were a linear relationship between the RRS intensity and the antibiotics concentration in the range of ,0.2-8.0μg·mL-1 for ETIS and 0.4 -8.0μg·mL-1 for NETS, respectively. In this dissertation, the spectral characteristics of the absorption and RRS, the optimum conditions of the reaction and the properties of analytical chemistry were investigated. In addition , the reaction mechanism and the influencing factors of the RRS spectra and the enhancement reasons of RRS have been discussed. |
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