Primary Observation Of Posterior Lens Capsule Stained By Trypan Blue In Vitrectomy

Purpose:To observe trypan blue staining of posterior lens capsule, and to explore thepossibility of trypan blue staining of the posterior lens capsule during vitrectomy.Method:60New Zealand white rabbits (120eyes) were randomly divided into3groups(group1,group2,control group),with each group20rabbits.Under the operatingmicroscope,lens has been removed completely. Anterior and posterior lens capsule wereteared off and put into trypan blue solutions of diffirent concentration.Light microscopyand electron microscopy were used respectively to observe the differences among theanterior and posterior lens capsule,datas were analyzed.Results:1．Staining results:In control group lens capsules were unstained and transparent. Inthe other two groups, anterior and posterior lens capsules were colored, and capsulastaining was darkened when the dye concentration increased.2．Light microscopy: In the control group, the surface of anterior and posteriorcapsules was smooth, uniform thickness, cell-free, and with no other covering. In theexperimental group, no significant change was found in the stained anterior and posteriorcapsules.3. Transmission electron microscopy: The surface of the unstained anterior andposterior capsular was smooth. Some scattered oval transparent areas not with uniformsize were seen in the capsular. After stained, we didn't find the transparent area in thecapsular, but some uneven larger particulate matter appeared on the surface. We called thechanges trypan blue positive.4．Statistical analysis:We counted the capsular particles which was trypan bluepositive in each group, then compared the experimental groups with the control group. The significant difference was found between the groups, P<0.05. And no significant differencewas found between experimental1and2groups, P>0.05.Conclusion:Low concentration of trypan blue solution may be safe and effective in posteriorcapsular staining.This method means a better prospect to use clinically to protect thetransparent lens during the vitrectomy.