| At present, drug-resistance has been a worldwide problem. Pathogens which have resistance to antibiotics emerged widely in the hospitals and communities. Because the generation of drug-resistance has many factors, multi-resistant organisms have emerged and diffused rapidly. The research of drug-resistance has become the focus in the world because the generation and diffusion of drug-resistance bacteria are rapidly. The Chinese drugs showed their powerfully vital force in resisting drug-resistance for their special theories and remarkable clinical curative effects. The components of Chinese drugs are complex. Bacterial resistance to them is not easily generated. And many Chinese drugs have excellently inhibitory activity.In the paper, drug-resistance of E.coli to four kinds of Chinese drugs was studied by microcalorimetry. The difference of drug-resistance of E.coli to the different active components of Chinese drugs was compared.These main contents are as follow:1. The first chapter summarizes research status about drug-resistance and Chinese drugs, development and actuality of thermokinetics and microcalorimetry, and introduces the chief contents and study significances of the paper.2. The extraction and characterization of the components of Chinese drugs According to the natures of different Chinese drugs, we adopted differentexperimental methods to extract the active components of them and got four kinds of monomers which were characterized by X-ray diffractometer, HPLC and FTIR.3. The Study of the regularity of E. coli growing and metabolizingIn order to fix the regularity of E.coli growing and metabolizing and the best concentration of drugs, we determined the P-t curves of E.coli growing and metabolizing at different concentrations of drugs. We got the growth rate constants at different concentrations of drugs by using the Logistic equation to deal with the curves. Then, we got the correlation between growth rate constants and the concentrations of drugs and fix the MIC (minimal inhibitory concentration). The results showed that the four kinds of monomers could all resist the E.coli quite well.4. The study of the regularity of the drug resistance of the E.coli to Chinese drugsThe E.coli was incubated in the SMIC .The power-time curves of the concentration for each incubated E.coli were determined. Based on the data of power-time curves and logistic equation, the growth rate constants (μ) were calculated. The relationship between μ and N was established. The regularity of resistance to Chinese drugs was obtained primarily. The results showed that the E.coli could generate the drug resistance bateria in the four kinds of drugs. The drug-resistance strengthened when the N increased.5. The study of the regularity of the drug-resistance decrease of the E.coli to Chinese drugsThe drug-resistance bacteria were incubated in the substrates. The power-time curves of the SMIC for each incubated E.coli were determined. Based on the data of power-time curves and logistic equation, the growth rate constants (μ) were calculated. The relationship between μ and I was established. The regularity of resistance decrease to Chinese drugs was obtained primarily. The results showed that with the increase of I, the drug-resistance of the drug resistance bacteria decreased even disappeared.The innovation of this paper:1. There are many researches on the drug resistance of bacteria to antibiotics. But the researches on the active components of Chinese drugs are few. In this paper, the monomers were extracted from the four Chinese drugs. And the monomers were characterized by X-ray diffractometer, HPLC and FTIR. 2. Microcalorimetry as a new method in studying drug resistance can measure correctly MIC. And the microcalorimetric method has the merits of rapid measurement, quantitative determination, reliable results, good repeatability. The drug resistance of E.coli to the monomers was studied. Some important parameters were obtained, which can provide academic bases for clinic treatment. |
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