The Biological Activity Of Flavonoids From Chinese Herbal Medicine And Bacterial Resistance Study

The natural medicine resources of our country are abundant,it causes the attention of the domestic and international scientist to draw the effective composition and develop specific drug from traditional Chinese medicine,make domestic and international scientists cast the sight that expects on traditional Chinese medicine.The developing focuses of a lot of scientific research institutions are herbal medicines to treat tumor,cardiovascular vessel disease,nerve spiritual disease,AIDS at present,it is a quite valuable scientific research task to look for the natural active substance to serve human health from it.Because the herbal composition is complicated, employ and separate technology modernly,draws active composition among them;can offer the data worth consulting for further developing utilizing.At present,drug-resistance has been a worldwide problem which has already caused the high concern.After studying in the active composition falconoid material from Chinese herbal medicine which can restrain the bacterium growth metabolism,the results showed that the S.aureus could generate the drug-resistance bacterium which can recover to some extent after being incubated in the substrate.This text has drawn the active composition of falconoid from 5 kinds of Chinese herbal medicines,then employs the Microcalorimetry to determine the thermal power-time curves, analyze the thermal power-time curve;Study of the regularity of S.aureus growing and metabolizing;Study of the regularity of the drug-resistance decrease of the S.aureus to Chinese drugsThe thesis includes six chapters:Chapter one:Foreword part,has introduced the overview of traditional Chinese medicine and falconoid material systematically;Have summed up the development of the Microcalorimetry and current situation of study on bacterium;Have introduced the little living beings and it's grown model;Discussion bacterium drug-resistance phenomenon.Introduce the chief contents and study significances of the paper.Chapter two:Draw the main active composition Purring monomer from Radix Pereira,has signified,study its bacteriostasis on the S.aureus.The change law of the speed constant grown by the bacterium draws the conclusion:Purring inhibits the growth of the S.aureus,fix the MIC (minimal inhibitory concentration)is 0.02433g/mlChapter three:Extract the glycoside activity monomer of ivies while doing from penetrating, signify,and study its bacteriostasis on the S.aureus.The result indicates,glycoside of ivies produces inhibitory activity on the growth of the S.aureus,the MIC(minimal inhibitory concentration)is 0.04922g/mlChapter four:Draw the total falconoid from the mulberry,study its bacteriostasis on the S.aureus.The result indicates the total falconoid of the mulberry will promote the growth of the S.aureus within certain concentration range,as the concentration strengthens,produces the bacteriostasis slowly;the MIC(minimal inhibitory concentration)is 0.01014g/ml.Chapter five:Draw total falconoid from the leaf of the bamboo,study its bacteriostasis on the S.aureus.The power-time curves were analyzed by using logistic equation;determine the growth rate constant of S.aureus.The relationship between growth rate constant and concentration was found.The MIC(minimal inhibitory concentration)is 0.015466g/ml.Chapter six:Draw total falconoid from Herbal Houttuyniae,study its bacteriostasis on the S.aureus.Analyze and obtain:Herbal Houttuyniae total falconoid inhibits the growth of the S.aureus,the MIC(minimal inhibitory concentration)is 0.009918g/ml.The conclusion has happened in the discussion of the S.aureu's drug-resistance:own to posterity increase of number of times with bacterium,the posterity's growth speed constant large slowly,prove five Chinese herbal medicine inhibitory activity in bacterium more and more little,bacterium produce drug-resistance to falconoid content that draw from the Chinese herbal medicine.Compare,go down to posterity fungus and produce the growth speed constant among medicinal solution of bacterium able to bear nature originally while being the tenth generation of through train among blank culture medium subsequently,find the drug-resistance decrease. medicinal solution has resumed restraining the fungus activity to it.The innovation of this text:Microcalorimetry is a new method in studying bacteriostasis and drug- resistance;can fix the MIC(minimal inhibitory concentration)correctly.This method has the merits of rapid measurement,quantitative determination,reliable results,and good repeatability.Some important parameters were obtained,which can provide academic bases for clinic treatment and apply the falconoid.