Cordyceps Mycelium Powder Control Manshuriensis Kidney Damage Renal Interstitial Fibrosis In Rats

Objective:To establish a model of Aristolochia manshuriensis Kom. decoction inducechronic renal failure in rats and to investigate the pathogenesis of its renalinterstitial fibrosis include alleviate its renal injury by culturedcordyceps sinensis powder.Methods:Thirty male Wistar rats were randomly distributed into three groups.Groupl(n=10) was normal control, Group 2(n=10) was model control, Group 3(n=10)was treated group. Group 2 was received Aristolochia manshuriensisKom. decoction 10g/kg body weight, Group 3 was received AristolochiamanshuriensisKom, decoction 10g/kg and cultured cordyceps sinensis powder5g/kg body weight once daily for 8 weeks. After administration, urine sampleswere obtained forβ₂-MG and 24 hours urine protein quatity(24HUPQ). In thehistological researches, Tubulointerstitial histological points,Tissueinhibitor of metalloproteinase-1 (TIMP-1) and Matrix metallo proteinase-9(MMP-9) were determined.Results:It was found that Protein of 24 hours urine andβ₂-MG of the group 2 and3 were higher than groupl (P＜0.05, P＜0.01). There for, the results of studysuggest that Aristolochia manshuriensis Kom. decoction could result intubulopathy. We also found that Group 3 resulted in improved 24HUPQ andβ₂-MGat points compared with Group 2, but the two had no difference with significance.There for, the results of study suggest that we need more times to make modelto confirmthe function of cultured cordyceps sinensis powder on alleviatingthe renal injury of rats which Aristolochia manshuriensis Kom. Decoction induced.In the histological researches, it was found that in the group 2 and 3the tubulo -interstitiaI histological points increased compared with those ofthe normal control (P＜0.01). The points of Group 2 was higher than Group 3, butthe two groups had no difference with significance (P＞0.05). The expressionof TIMP-1, MMP-9 in the tubular ceils increased compared with those of the normalcontrol (P＜0.01). There for, Aristolochia manshuriensis Kom. decoction couldstimulate the expression of MMP-9 in the renal tubular epithelial cell, becauseof it increase the synthesize of ECM, at the same time the downgrading reactionis active. Later the expression of TIMP-1 is increased because of feedbackmechanism. At last, MMP-9/TIMP-1 lost of its balance to restrain thedegradationof collagen and aggravate tubulointerstitial fibrosis. We also found that inthe group 3 the expression of TIMP-1 in the tubular cells increased comparedwith those of the cultured cordyceps sinensis powder treated (P＜0.01) whilethe expression of MMP-9 decreased. There for, the results of study suggestthat cultured cordyceps sinensis powder could restrain the expression of TIMP-1in the tubular cells and alleviate tubulopathy by decreasing the sludging ofECM.Conclusion:The results showed Aristolochia manshuriensis Kom. decoction could resultin tubulointerstitial fibrosis. Because of increasing the synthesize of ECM,at last MMP-9/TIMP-1 lost of its balance which induce tubulointerstitialfibrosis and activate renal injury. Cultured cordyceps sinensis powder coulddecrease urineβ₂-MG and the 24 HUPQ, but the two had no difference withsignificance. There for, the results of study suggest that we need more timesto make model to confirm the functionof cultured cordyceps sinensis powder on alleviating the renal injury of rats which Aristolochia manshuriensis Kom.Decoction induced. Cultured cordyceps sinensis powder could decrease theexpression of TIMP-1 and up the expression of MMP-9. With respect to potentialtherapeutic implications of the findings, it is of crucial importance toinvestigate the effective mechanisms of cultured cordyceps sinensis powder onrenal failure which Aristolochia manshuriensis Kom. decoction induced.