Ditan Decoction On Learning Of The Rat Model Of Alzheimer's Disease And The Cholinergic System

ObjectiveThis experiment in old rats forebrain nuclear injection IBO and Aβ_25-35mixture of compound-induced animal model of AD observed Ditan decoction ratmodel of AD behavior and the central cholinergic system, Discussion Ditan treatingAD mechanism for the treatment ofAD Ditan decoction provide a scientific basis.Method5-month-old Wistar rats 50, half male and half female, were divided into fivegroups: normal, sham-operated group, model group, Huperzine A group and Ditandecoction groups, each 10. Rat forebrain nuclear injection and IBO Aβ_25-35 mixtureof compound-induced animal model of AD. Animal Ditan decoction and Haberintervention by 28 days, the rats with Morris water maze behavioral experiments(including positioning navigation experiments and experimental search space of twoparts) Frederick Measuring learning and memory in rats and usedassayed.Meanwhile rat hippocampus and cortex Ach, AchE and ChAT activity.Results1. Experimental rats navigation positioning before the average incubation periodis four clays after two days and the average incubation period showed : model grouplatency than other groups significantly prolonged compared with the normal grouphad significantly (P＜0.01) Huperzine A Group and the group Ditan decoctionsignificant differences (P＜0.01). Huperzine A group Ditan Decoction four daysbefore the incubation period more significant difference (P＜0.01) Ditan Decoctionless than Huperzine group, after two days of incubation period (P＞0.05). Normalgroup and sham surgery group (P＞0.05).2. Rats positioning navigation experiments show: the model group quadrantpercentage reduction, 20% and 40% of the regional regional significantly increasedCompared with the normal group had significantly (P＜0.01) Huperzine A and Group and the group Ditan decoction significant differences (P＜0.01). Huperzine A groupDitan decoction regional group 40% significant differences (P＜0.01) DitanDecoction short of Huperzine A group quadrant% and 20% no regional difference(P＞0.05). Normal group and sham surgery group (P＞0.05).3. Spatial search results show: the model group quadrant% compared with theother groups decreased, and 20% and 40% of the region there is a marked increasefrom the region, compared with the normal group had significantly (P＜0.01)Huperzine A and Group and the group Ditan decoction significant differences(P＜0.01). Huperzine A group Ditan decoction regional group is 20% (P＜0.01) DitanDecoction less than Huperzine A group quadrant% and 40% no regional difference(P＞0.05). Normal group and sham surgery group (P＞0.05).4. In the original platform location through the number of experiments show:the original model through the platform location other than the obvious number ofthe small group, Compared with the normal group had significantly (P＜0.01).Huperzine group and Ditan decoction with model group compared with a significantdifference (P＜0.01) normal group and sham group showed no significant difference(P＞0.05), Huperzine A group Ditan Decoction group was no significant difference(P＞0.05).5. Rats central cholinergic system experiments show: model Ach brain tissueand reduce the activity of CHAT, AchE activity was significantly higher, comparedwith the normal group had significantly (P＜0.01) Huperzine A and Group decoctionand Ditan group Ach, CHAT activity was significantly reduced, AchE activityincreased significantly, statistical significant difference (P＜0.01). Ditan DecoctionAch, ChAT activity and Huperzine group, a statistical difference (P＜0.05) AchEactivity of the two groups showed no difference (P＞0.05). Normal group and shamsurgery group (P＞0.05). Conclusion1. Aged rat basal forebrain nuclei injection IBO and Aβ_25-35 mixture ofcompound-induced Alzheimer's disease rats, performance of the learning andmemory abilities significantly lower Ach, ChAT activity decreased significantlyincreased activity of AchE, The model embodies the characteristics of the AD is amore reliable animal model of AD.2. Ditan decoction can be significantly improved AD rat model of learning andmemory impairment.3. Ditan decoction can enhance brain tissue Ach and the expression of CHAT,reducing the activity of AchE. the cholinergic nervous system damage protectiverole.4. Chinese AD incidence of sputum and development is closely related to DitanResuscitation control is the basic rule AD, Ditan decoction Treatment AD efficacy.