June Green Petroleum Ether Extract Of S180 Mice Inhibitory Effect

It has broad prospect from traditional Chinese medicine to find active ingredients for antitumor.Liuyueqing is widely distributed in Guangxi. Pharmacological research confirme that Liuyueqing has antivirus effect.Some preliminary experiments prove Liuyueqing extracts have antitumor activity. However,systematic study of antitumor activity on Liuyueqing is almost blank at the present.In this experiment,we study the antitumor activity of extract from Liuyueqing to produce the scientific evidence for its further research and exploitation.Objective:To study the inhibitory effect and its mechanism of LYQPE on transplanted S₁₈₀sarcoma model in mice.Methods:(1)Transplanted S₁₈₀sarcoma in mice was used as a model:50 mice were inoculated subcutaneously with 0.2 ml of S₁₈₀sarcoma cell in right axilla.24 hours afher inoculation,these mice were randomly divided into five groups:tumor model group,CTX positive control group,petroleum ether extract of LYQ(LYQPE)high-dose group,middle-dose group and low-dose group(n= 10).All mice were treated with drug after 24 h after inoculation for 10 days. Blood was collected,and mice were sacrificed 24 h after last dose of drug. Tumor tissues were weighed,and inhibitory rate of tumor was calculated.(2) Effect of LYQPE on immunological function:thymus and spleen of mice of transplanted S₁₈₀sarcoma were culled and weighed.Index of thymus and spleen were calculated.Spleen lymphocyte suspension was prepared and stimulated by Con A.Proliferated ability of lymphocyte was assayed by MTT method. Macrophage suspension was prepared and it's the phagocytized ability of macrophage was assayed by neutral red method.The content of tumor necrosis factor-a(TNF-α)and interleukin-2 were assayed by ELISA.(3)Anti-oxidative effect of LYQPE:The content of maleicdialdehyde(MDA)and superoxide dismutase(SOD)were assayed by colorimetry.(4)Effect of LQYPE on pathological change in S₁₈₀tumor tissue:tumor tissue was fixed by formaldehyde,and dyed by HE stain and pathological changes were observed by light microscope,and P₅₃protein expression in tumor tissue was determined by immunohistochemical method.Results.(1)LYQPE could inhibit the growth of transplanted S₁₈₀sarcoma in mice.Inhibitory rates in high-dose group;middle- dose group and low-dose group were were significantly different from that in model group(P＜0.01 or P＜0.05).The inhibitory rates of high-dose group and middle-dose group were more than 30%.(2)LYQPE could increase the indexes of thymus,which in high-dose group was increased significantly(P＜0.05),but LYQPE had not influence on the indexes of spleen of mice with S₁₈₀tumor(P＞0.05).LYQPE could stimulate lymphocyte proliferation,which in high-dose group and middle-dose group were significantly different from that in model group (P＜0.01).LYQPE could increase phagocytized ability of macrophage,which in high-dose group and middle-dose group was significantly different from model group(P＜0.01 or P＜0.05).LYQPE could increase the content of tumor necrosis factor-a,which in high-dose group and middle-dose group were significantly different from that in model group(P＜0.01 or P＜0.05).LYQPE could increase the content of interleukin-2,which in high-dose group,middle-dose group and low-dose group were significantly different from that in model group(P＜0.01). (4)LYQPE group could decrease the content of MDA,and increase the activity of SOD,which in high-dose group,middle- dose group and low-dose group were significantly different from that in model group(P＜0.01 or P＜0.05).(5) Acellular necrosis of tumor tissue was more serious in high-dose group and middle-dose group that in model group.LYQPE could decrease mt P₅₃protein expression,which in igh-dose group,middle-dose group and low-dose group were significantly different from model group(P＜0.05).Conclusions:LYQPE can inhibit the growth of transplanted S₁₈₀sarcoma in mice,and the increases in the immunological function,antioxidative ability and induced apoptosis of tumor cell by LYQPE were the possible action mechanism.