Four Gentlemen Decoction Resistance To Chemotherapy Drugs Cyclophosphamide Mutagenicity Experiments

Objective: To systematically research the ant mutagenesis, dosage effect and themechanism of SiJunZi decoction, to approach the protection of genetic material. Thismaybe can provide experimental evidences for SiJunZi decoction's clinicalapplication.Methods: Three tests were used in this topic, that is, mouse bone marrow cellmicronuclei and chromosomal aberration test, human body peripheral bloodlymphocyte micronuclei test and chromosomal aberration test. In each test, there werefive groups. They were blank control group, positive control group, SiJunZi decoctionhigh dose group, SiJunZi decoction middle dose group, and SiJunZi decoction lowdose group. The positive control drugs are Fluorouracil, arsenic trioxide andcyclophosphamide. Preparations were prepared. They were observed and count underlight microscope double-blinded. The typical samples were taken photos by camerathrough ocular. All the data were statistic by t-test.Results1 Results of mouse bone marrow cell micronucleus test: Compared with blank group,micronuclear rates of positive group (CPP, 5-FU, As₂O₃) is obviously higher thanblank group's. If 5-FU is positive control, the micronuclear rates in low and middledosage of Sijunzi decoction is lower than positive group (P＜0.05), and the highdosage of Sijunzi decoction's is obviously lower, there is significantly differentbetween them (P＜0.01). If As₂O₃ is positive control, the micronuclear rates in low andhigh dosage of Sijunzi decoction is lower than positive group (P＜0.05), and themiddle dosage of Sijunzi decoction's is obviously lower, there is significantlydifferent between them (P＜0.01). If CPP is positive control, the micronuclear rates inmiddle and high dosage of Sijunzi decoction is lower than positive group, there issignificantly different between them (P＜0.01). Analysis with ANOVA, in the test of antimutagenesis of sijunzi decoction, dose-effect relationship is not exists.2 Results of mouse bone marrow cell chromosomal aberration test: Compared withblank group, the aberration rate of positive group (CPP,5-FU,As₂O₃) is obviouslyhigher than blank group's. If 5-FU is positive control, the aberration rate in low andmiddle dosage of Sijunzi decoction is lower than positive group (P＜0.05), and thehigh dosage of Sijunzi decoction's is obviously lower, there is significantly differentbetween them (P＜0.01). If As₂O₃ is positive control, the aberration rate in low andhigh dosage of Sijunzi decoction is lower than positive group (P＜0.05), and themiddle dosage of Sijunzi decoction's is obviously lower, there is significantlydifferent between them (P＜0.01). If CPP is positive control, the aberration rate inmiddle and high dosage of Sijunzi decoction is lower than positive group(P＜0.05).Analysis with ANOVA, in the test of antimutagenesis of Sijunzi decoction,dose-effect relationship is not exists. Analysis with linear correlation, there issignificant positive correlation between micronuclear rates and aberrationrate(r=0.920993, p＜0.01).3 Results of peripheral blood lymphocyte of human body's micronucleus test:Compared with blank group, the micronucleus rates of positive group (CPP, 5-FU,As₂O₃) is obviously higher than blank group's. If 5-FU and As₂O₃ is positive control,the micronucleus rates in low dosage of Sijunzi decoction is lower than positive group(P＜0.05), and the middle and high dosage of Sijunzi decoction's is obviously lower,there is significantly different between them (P＜0.01).If CPP is positive control, themicronucleus rates in middle dosage of Sijunzi decoction is lower than positive group(P＜0.05). The high dosage of Sijunzi decoction's is obviously lower, there issignificantly different between them (P＜0.01). Analysis with ANOVA, in the test ofantimutagenesis of Sijunzi decoction, dose-effect relationship is not exists in As₂O₃and CPP groups, but there is dose-effect relationship in 5-FU group.Conclusions1 SiJunZi decoction.shows its ant mutagenic activity not only in vivo but also in vitro.2 The possible mechanism of SiJunZi decoction's ant mutagenesis were strengthen protection of genetic material and repair capability of damage on the chromosomelevel.3 SiJunZi decoction can protect genetic material of human and mouse to a certaindegree.