Peony Root. Wine Processing Technology, Quality Control And Its Pharmacodynamics

Studies on processing technology,quality standard and efficacy of Paeonia lactiflara Pall processed with wine came from a section of research contents related to the Project of Hubei Genuine Medicinal Materials. This project was conducted by the theory of traditional Chinese medicine, which made a modification and testification of Paeonia lactiflara Pall processing, applied with modern sci-tech and methods, in order to ensure the safety and stability and the author has made an experimental study on the efficacy of Paeonia lactiflara Pall processed with wine.1. Study on Summary of literatureThe article made an inducement and arrangement of Paeonia lactiflara Pall processing's related contents such as originals,development,current conditions of processing and traditional processing theory, in order to supply further study on Paeonia lactiflara Pall processing with reference .There was almost 50 papers recorded Paeonia lactiflara Pall processed with wine. Modern literature indicated that Paeonia lactiflara Pall contained paeoniflorin and other different compounds, which has multi-pharmacological action such as sedation,spasmolysis,antiinflammatory and dilating coronary artery.2. Study on processing of White Peony RootIn the point of processing technology, we used the contents of water-soluble extract and ethanol-soluble extract as evaluating indexes and investigated the test. The optimum technology of softening and drying,individually were steaming-soaking with water for 3-5 hours and then steaming, and slicing on-line, and temperature 60℃,thick 2cm,evenly drying 40min and turn over once. In addition, we used the content of paeoniflorin as evaluating indexes and investigated the test. The optimum processing technology with wine was, the amount of alcohol 10%, temoerature 90℃, processing 10min.3. Study on quality standard 3.1 The White Peony Root processed with wine was identified by TLC, and results said that, the spot of all same color , in providing sample chromatography and in comparing medicinal materials' corresponding position without negative interference.It was verified that, clear spot,good repeatability and strong specialization.3.2 The method of determining the amount of paeoniflorin in White Peony Root processed with wine was developed by High Performance Liquid Chromatography. The chromatographic condition was Agilent C18 column, the mobile phase was acetonitrile-water(17:83), the flow velocity was 1.0ml/min, the detecting wavelength was 240nm, the column temperature was 25℃.The standard paeoniflorin showed a good linear correlation within the concentration of 0.0604～0.8456μg (r=0.9995) .The average recovery rates were 99.49%, respectively while RSD was 0.46%. Assay methods were validated in the research of content. On the basis of the above-mentioned quantitative analysis methods for paeoniflorin, the quality standard of White Peony Root processed with wine was drafted.4. Study on efficacyWe choosed the experiments of anticoagulation,analgesia,antiinflammatory and sedation to observe the changes of the medicine between original and processes in Pharmacology.①From anticoagulation experiment, we obtained that medicine processed with yellow wine had stronger pharmaceutical effect than its original in the three aspects.②From experiment of mice tail thermal shrinkage, we found that the processed was stronger in analgesia.③From experiment of mice ear swelling caused by dimethyl benzene, the result told us that the processed with wine and the original had antiinflammatory effect without sharp differences.④From motionless time experiment of mice swimming by forcing, we found that the processed was stronger in sedation.