.660 Nm Low Level Laser Irradiation Rabbit Erythrocyte Hemolysis Effect

Bio-effects of low intensity laser radiation are interdisciplinary research fields, which is of important prospect and wide attention.The hemolysis of rabbit's erythrocyte in vitro under irradiation of 660nm semiconductor laser was investigated. Under certain conditions, the results showed the hemolytic rate is increased. It suggested that erythrocyte is the target cell and sensitive to low intensity 660nm laser, which produced notable bio-effects.The rabbit's fresh erythrocyte suspension washed by physiological saline is the object to be observed, sodium heparin solution is the anticoagulant. The ratio of erythrocyte hemolytic rate of irradiation group and control group will be investigated.Experimental result indicated that low-intensity semiconductor laser irradiation enhances the ratio of erythrocyte hemolysis; the effect is time-delayed (There was no significant difference between the test and control groups when tested in two hours, but after 24 hours the hemolysis of control group is prominent). Hemolysis is positive correlation with laser power; polarized light has no significant effect on hemolytic rate. When the laser power is similar, the hemolytic rate of 660nm semiconductor laser is significantly lower than 632.8nm. He-Ne laser. In the same condition, hemolytic rate marked individual difference. Erythrocyte suspension with blood preservation solution has lower hemolytic rate than which without it.This research may offer new experimental support for the study of bio-effects of low intensity laser radiation and its mechanism. Then impact factor and biological significance are analyzed. The new idea that erythrocyte endogenous porphyrin makes photochemical reaction under laser irradiations and lead to biological effects is proposed.The result of this paper will provide important experimental support and theoretical guidance for the study of mechanism about photo-blood interaction, low-intensity laser biological effect and low-intensity laser therapy. The research method of this paper may provide a new way to study the low-intensity laser biological effect.