Effect Of Low Intensity He-Ne Laser On Erythrocyte Deformability And Ultrastructure Of Erythrocyte Membrane

In this thesis, we mainly investigated the influence on erythrocyte deformabilityirradiated with different doses of low intensity He-Ne laser by nucleopore filter filtration, andthe effect on ultrastructure of human erythrocyte membrane irradiated with low IntensityHe-Ne Laser by atomic force microscope.First of all, the paper introduced the biostimulation mechanism of low-intensity laser andsummarized the actuality and brief history of the action of blood and erythrocyte bylow-intensity laser. At the same time, this paper explained the aim and significance of the study.And then this paper recapitulative introduced the system and application of atomic forcemicroscopy.The effect of low intensity He-Ne laser has already been ratified widely. A lot of studiesused biochemistry data to show the effect of low intensity laser radiation on erythrocyterheology. Despite of numerous reports on laser cellular effects, the interaction mechanisms oflow intensity laser irradiation with erythrocyte and its components are still far from to be clear.There is little report about the molecule images of the normal or damaged erythrocytes afterirradiated by laser. To find out the mechanism of modulating the deformability of erythrocyteas a result of low intensity He-Ne laser action, we mainly investigated the influence onerythrocyte deformability irradiated with different doses of low intensity He-Ne laser and theeffect on ultrastructure of human erythrocyte membrane irradiated by low intensity He-Nelaser from the point view of molecular images by atomic force microscope. The results of thisresearch will provide valuable reference for the theoretical mechanism of modulating thedeformability of erythrocyte by laser irradiation. After repeated experiments, we got someresults as the following.After treated with free radical, the deformability of damaged erythrocytes weresignificant decreased compared with normal erythrocytes. But damaged erythrocytesirradiated by different doses of low intensity He-Ne laser, the deformability of erythrocyteswas obviously better than that of non irradiated. No obviously doses-effect of low power laseron erythrocyte deformability was showed in this experiment. AFM images of the whole erythrocyte, revealed that there was no difference in thewhole shape aspect, except the deepness of central concave area between the damagederythrocyte and the normal one. However, the surface of normal erythrocyte membrane wassmooth, while the surface of the damaged erythrocyte was very rough. AFM images of theultrastructure of the membrane of damaged erythrocyte treated with free radical, showed thatthe molecules on the surface of damaged erythrocyte membrane congregated in particles ofdifferent magnitudes. Comparing the degree of congregation of the protein molecular particlesin the non-irradiated group and the He-Ne laser irradiated group (9mW and 18mWrespectively), we found that it had been improved. The average size of molecular particles inthe laser irradiated group was smaller than in the non-irradiated group. Moreover, Thefluctuation curves of erythrocyte membrane with irradiated group were much flatter than thatof the non-irradiated. The distance between peaks in irradiated sample was smaller than innon-irradiated sample. To some extent, there was a repair function of low intensity laser withregard to the damage produced by the free radicals on the erythrocyte.Last, paper prospected the foreground of the application of AFM in the field of life scienceand low-intensity laser treating sickness in clinic.