Houttuynia Injection Fingerprint And Anti-inflammatory, Antibacterial Efficacy Component Of The Study

In the process of improving quality appraisal system for traditional Chinese medicine(TCM),fingerprint technique turns to be a practical and feasible mode to maintain the stability of product quality.And it can better distinguish the authenticity of TCM.The brought up of fingerprint technique promotes a development in research and quality analyzing method from the study for one or several active ingredients to a comprehensive study for the whole TCM.This thesis sets out to settle the problem of chromatographic fingerprint research of TCM injection:the following work was done with hyphenated chromatographic instruments and chemometric approaches:the study of the correlation between fingerprint and anti-inflammatory effect and inhibitory activity.The first attempt on fingerprint-efficacy of Houttuynia cordata injection was developed.The fingerprints of Houttuynia cordata injection from different factories were classified by hierarchical clustering. Anti-inflammatory effect of Houttuynia cordata injection was characterized by the rat pleurisy model induced by carrageenin and the mice ear edema model by xylene.Viable count method was used to determine inhibitory activity of Houttuynia cordata injection. Anti-inflammatory effect and inhibitory activity of the injection from the first class factories on the two models were significant,while the second class were not.The analysis result indicated that main effect compounds in Houttuynia cordata injection are methyl n-nonyl ketone, decanoylacetaldehyde,lauryl aldehyde,capryl aldehyde,β-pinene,β-1inalool,1-nonanol,4-terpineol,α-terpineol,bornyl acetate,n-decanoic acid and acetic acid,geraniol ester etc.The author then established a corresponding relationship between the fingerprint of Houttuynia cordata injection and efficacy to certain extent. The pattern of Houttuynia cordata injection is based on the efficacy.The mode established in this thesis provides specific experiment data and also a solid foundation for the establishment of Houttuynia cordata fingerprint standards.