| Hydrogen sulfide is one of the three gaseous signal molecules together with NO (Nitric Oxide) and CO (Carbon monoxide). At present injury from ischemia-reperfusion (IR) is still the important syndrome and one of the lethal factors of coronary arterial recirculation, and cellular apoptosis has been proposed as a key process. So it was postulated that inhibition of apoptosis-related genes expression could alleviate the injury from IR.We observed the cardioprotective effects of exogenous H2S and investigated its possible cellular signaling pathways. The IR models were established on male Wistar rats (250-350g). Ten minutes before the ligation of left coronary artery, NaHS (donor of hydrogen sulfide, 30mmol/L, 40mmol/L, 50mmol/L) was injected through left femoral vein; thirty minutes later, clips were removed to reperfuse the rat heart for 2 hours. It was found that H2S could improve the hemodynamic parameters (LVEDP, LVESP, +dp/dt.max, -dp/dt.max, etc), reduce the size of myocardial infarction and prevent the apoptosis of cardiomyocytes. Also H2S could influence the protein levels of Survivin, Bax and phosphate-Gsk-3β, and the levels of Survivin was positively related to the cardioprotective effects of H2S. The relationship between the Survivin and the cellular signaling pathway of PI-3K/Akt/GSK-3βhas been tried to be established. The further studies on cellular signaling pathway need to be carried on cultured cardiomyocytes from neonatal rats. |
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