Road Estate Papaya Index Component Analysis And Sichuan Papaya Total Flavonoids Extraction And Purification,

Mugua is the dry and mature fruits of the Rose family plant chaenomeles(Sweet) Nakai,recorded in the China pharmacopoeia(2005 edition).It is a common Rheumatism Chinese medicine,which is sour in flavor,warm in nature,and attributive to the liver and spleen meridians.It is thought that the quality of mugua which from Anhui,Zhejiang,Hubei are the best.Sichuan has it largest production,but the quality research reports about Chuan mugua are very few.In order to appraise the quality of Chuan mugua,this research carries on the analysis to the different habitat mugua raw material for medicine target ingredient.Mugua includes the many kinds of flavonoids,extract total flavanone from it,not only for the flavonoids matter provides the new material,also may seek for the Chinese flowering quince industry to the new developmentspot.For further conducts its pharmacology research to lay the foundation.The comparison of organic acid and the triterpenoids acid between Chuan mugua and other different soursed mugua were researched.The extraction,purification of flavonoids in Chuan mugua was researched too.The main results are as follows:1.Different sourced mugua ingredients comparative analysis.The content of total organic acid of different sourse mugua were researched.The result is that the content of different Chuan mugua samples were all above 6%,and the result is to closede to Xuan mugua,except one sample which from Guanyuan.For the first time,The RP-HPLC was established for determinating the content of malic acid and citric acid in mugua simultaneously.There was good linearity between peak areas and injection quantity of malic acid range from 0.426～2.982ug and citric acid range from 0.164～1.148ug.The average recovery rate was 99.84%for malic Acid and 99.71%for citric acid.The meathod was reasonable,stable and accurate. The result of determination can be applied to judge the quality of mugua.The two kind of ingredients content of eight different sourced mugua were determined.The result is that the kind of ingredients content sum total all above 11mg/g. Ursolic acid and oleanolic acid as reference substance were determined by RP-HPLC method.The result is that the content of different samples are different.A spectrophotometric method was established for quantitative determination the total content of flavonoids in chaenomeles(Sweet) Nakai.The result showed that the optimal determination wavelength was 510nm.There was a good linear relationship between the absorbance and the quantity of rutin when the concentration of rutin(contrast) within the range of 4.64ug～27.84ug/ml.Regression equation was Y=1.0998X-0.0073 with the correlation coeffcient of 0.9991.The average recovery rates were 97.84%.This method is stable and precise as well as high recovery rates. We determine the total flavonoids content of eight different sourced mugua.The result is that the content of total flavonoids from Sichuan is as much as Anhui.4.The general inspection to the different habitat raw material for medicine has been carried,finally indicated raw material for medicine all conforms to the pharmacopoeia stipulation standard.2.The extraction,purification of flavonoids in Chuan mugua.The extraction process of the total flavonoids in Chuan mugua was investigated by single factor and orthogonal experiment.The optimum condition for the extraction of the total flavonids in it was that alcohol concentration is 80%;temperature is 60C;extraction time is three and one hour of each and the ratio of solid to liquid is 1:10.The column Chromatography was employed to enhance the content of the total flavonoids.In terms of the result of static adsorption and static de-adsorption test, D₁₄₀ resin was the best one for isolation flavonoids.The optimal adsorption parameters were feeding rate 1 BV/h,pH value 5～6,medicine liquid concentration 0.1mg/ml.The optimal de-adsorption parameters were eluted with distilled water, 3BV 10%ethanol and 3BV 50%ethanol in turn,flow rate 2BV/h.The purified total flavonoids was collected in 50%ethanol eluting solvent.