Compound Kushen Soothe The Nerves Particle Preparation Process, Quality Standards And The Correlation Of In Vivo Drug Research

Insomnia is more common in modern life,the type of disease,mainly manifested as difficulty falling asleep,wake up easily,wake up early,or poor quality sleep or decreased sleep time,or a combination of several.Western emphasis on regular rest and treatment of the rational use of sedative and hypnotic drugs,the clinical use of sleeping pills of choice,although the drug hypnosis faster,but there are considerable side effects, as well as drug resistance,addiction and so on,so patients often feel fear.Traditional Chinese Medicine has the basic roles of treatment of this disease in Liver ease lifting depression and the elimination of phlegm,and more to achieve the purpose of both temporary and permanent cures.This prescription derived from experience summarized by Chinese medicine doctor, and granule agent was made through modern technical method to cure a kind of hyptroplasia of mamma rygland in order to develop clinical effect and adapt to the need of market.1 Documents summariesStandpoint about Insomnia in Chinese medicine,the reason and mechanism,type and cure rule,Standpoint about Insomnia in west medicine and cure rule.clinical drugs in Chinese and west medicine.2 ExperimentsThe research of pharmaceutical including pharmaceutical technology,quality standard and drug-related in vivo.Pharmaceutical technology:We got the best prescription through acts of pharmacological tests;Orthogonal test was employed to determine the optimal condition.Granule was selected by virtue of theories of pharmaceutical technology design and characteristics of three Chinese medicine.The optimum technology as follows:Kushen(Sophora flavescens Ait),extracted 3 times by 12 times the volume of water,each time 0.5h,filtered,concentrated,concentrated decoction to the relative density of 1.05～1.10(50℃measured),the increase in ethanol concentration to 60 percent alcohol,place overnight,leaching,recovery of alcohol,concentration,vacuum drying.Pharmaceutical technology was determined after extraction.Quality standard research:The component was evaluated by TLC,The content of matrine and oxymatrine was determined through HPLC method.Drug-related in vivo:the main source and changes was determined through fingerprint comparison;the blood time of the peak in rats was determined by HPLC;the initial guess from basic information of blood components was determined by LC-MS study.The best extraction and pharmaceutical technology and quality standard was determined,conducted a preliminary study of drug metabolism in vivo,the initial guess from basic information of blood components was determined by LC-MS for further study.