Matsutake Extract Anti-tumor Effect

This paper extracts of matsutake macromolecular extraction,purification,then proceed to the effective components of the experimental anti-tumor research,determine the antitumor ingredients,reduce the dosage and to further expand,thereby to the development of new products.When Extraction,preceded by a number of single-factor test,then determine the water than expected,the extraction temperature,extraction time the three factors were orthogonal test.When the water reached to the best of conditions,the production and economic accounting.Final water than expected:1:30;extraction temperature:120℃;extraction time: 3h,the conditions,polysaccharides rate 8.12%.Purification,the system carried out from-protein.Sevag used,combining enzymatic Sevag,TCA,10,000 interception ultrafiltration membrane elements,anion and cation exchange resin method of removing protein.With weak basic anion and cation exchange resin weak acid for a rough series from polysaccharide protein,the effect has been very positive.The law under mild conditions, some bleaching effect and easy to industrialization,protein removal rate of 92.36%,82.85% for the recovery of polysaccharides,The molecular weight of 10,000 dialysis membrane dialysis interception,Freeze-drying Tricholoma matsutake polysaccharide obtained powder for yellow powder,Polysaccharide content of 89.27%,protein content is 4.55%.Polysaccharide ABMPâ… with matsutake,ABMPâ…¡intragastric administration,S₁₈₀ tumor in mice have some anti-tumor effect,Inhibitory effect of which ABMPâ… ABMPâ…¡than good. Two components of the mouse S₁₈₀ tumor dose-dependent inhibition of both Gavage dose of 20mg/kg.d,ABMPâ… the inhibitory rate of 46.3 percent:presented,ABMPâ…¡the inhibitory rate of 38.7%.Tricholoma matsutake polysaccharide ABMPâ… ,ABMPâ…¡intragastric administration, Liver cancer in mice ascites tumor entities better anti-tumor effect,Inhibitory effect of which ABMPâ… ABMPâ…¡than good Two components of the mouse liver cancer ascites tumor inhibition entities have all appeared dose-related,Gavage dose of 200mg/kg.d,ABMPâ… the inhibitory rate of 53.4 percent;ABMPâ…¡the inhibitory rate of 40.1%.By intragastric administration,Tricholoma matsutake mycelium crude polysaccharide and matsutake Exopolysaccharides S₁₈₀ tumor in mice have good inhibitory effect.Tricholoma matsutake mycelium coarse polysaccharide gavage dose of 500mg/kg.d,Inhibition rate was 52.8%;Tricholoma matsutake Exopolysaccharides gavage dose of 300mg/kg.d,Tricholoma matsutake Exopolysaccharide inhibition rate was 54.6%When gavage dose increased,but the inhibition rate drop,In the high-dose,the Tricholoma matsutake extracellular polysaccharides on the immune system in mice inhibited.