Photo Tears Only Congested Nose Soup Kidney Yang Experimental Allergic Rhinitis Serum Cytokines Il-12, The Expression Of Gm-csf,

OBJECTIVE:To study the effect of Sheti-Zhiqiu decoction to the level of serum IL-12 and GM-CSF of rats and pathology changes of Allergic rihinitis(AR) in Kidney-Yang deficiency type,we explored the mechanism of Sheti-Zhiqiu decoction in treating AR from pathology, physiology,biochemistry and molecular biology.We are going to establish the theory basis of clinic treatment—supplement Qi for strengthening exterior and warm Yang to dredge the orifices—of AR through the research in our lab.METHODS:Establishing rat's AR model in Kidney-Yang deficiency type,2 groups were involved which are the normal group and the model group.Establish the rat's Kidney-Yang deficiency model by the combination of the subcutaneous injection with Hydrocortisonum accoding to Miao Minsan report and the threat from cat and men,AR modal according to Feng Xuemei's.After the model was eatablished,the model group was divided into 5 groups randomly including Kidney-Yang deficiency AR group,the control group of Ketotifen,the high dosage group,the middle dosage group and the low dosage group of Sheti-Zhiqiu decoction.Then,Sheti-Zhiqiu decoction or Ketotifen was given to the rats by enema,starting from 36rd days to 42th days.During the treatment phase,the rats were stimulating by 2%OVA 1 hour after the medicine administrate, the author observed the symptoms especially nasal symptoms. After treatment,author choice 6 rats to apply the Light microscope to observe the pathogenic changes happened in the model rats's nasal septum,examining the level of IL-12 and GM-CSF in blood with ELISA.RESULTS:Serum IL-12 of rats low down in the modal before treatment(P＜0.01,compare to the normal group),and arise after treatment(P＜0.05 or P＜0.01,compare to the Kidney-Yang deficiency AR group).Serum GM-CSF of rats arise in the modal before treatment(P＜0.01,compare to the normal group),and low down after treatment(P＜0.05 or P＜0.01,compare to the Kidney-Yang deficiency AR group).All the treatment groups and the normal group have no differences in serum GM-CSF.CONCLUSION:One of the therapeutic mechanism of Sheti-Zhiqiu decoction is releasing the pathology changes of the rat's noses and regulating the level of serum IL-12 and GM-CSF.