Puerarin Injection On Acute Retinal Light Damage Of Inos, Of Cyt-c Expression

Objective:To observe the morphologic,biochemical and electrophysiologic changs of the retina in rats after exposure to the light of the operation microscope,and study the protective effect and mechanism of Pue against photic injury of retina.Methods:The experiments were performed on fouty—eight healthy Sprague-Dawley rats which were devided into four groups—normal group,model group,high-dose group and low-dose group.All the groups except the normal one were continually exposed to the light of the operation microscope with an illuminace level of 22000±1000lux for 30 minutes.The treated received daily Pue for one week after exposure to the light,then,the pathological changes of retina were examined by light microscopy;and INOS,Cyt—c and Caspase-3 into retina were tested.Result:①After the light exposure,the outer nucleus layer of retina in the model group became thinner and the regular arrangement of outer and inner segments were disordered,dividing line wasn't distinct.In the low-dose group the outer nucleus layer of retina got thicker than that of the model group.②Pue can adjust and control the gene expression in phototonus of retina.Conclusions:Pue could release the photic injury of retina,accelerate the renovation of retina after light exposure and ameliorate the function of retina. The mechanism might relate to alleviate the poisonous effect of retina that nitric oxide result in,control the gene expression in phototonus of retina.