Renal Blood Ning Decoction On The Organization Of Primary Iga Nephropathy Of Type Iv Collagen In Rats

Goal:Through Shenxuening Jianji to primary IgA nephrosis big mouse's kidney organization in typeⅣCollagen expression influence, discusses it to the primary IgA nephrosis kidney fibrosis influence and the action mechanism, seeks the effective medicine to postpone or prevent the IgA nephrosis course development.Method:Body weight 160±20g healthy female Wistar big mouse 48, before the experiment, makes the urine protein, the urine occult blood inspection, removes the non-health experimental animal to the experimental result influence. Divides into 4 groups stochastically, namely blank control group, model group, Shenxuening Jianji group, Diovan (Valsartan) group, each group of 12. Selects 3 groups stochastically, establishes the IgA nephrosis model. 12 weeks later, observes the indicators about the big mouse 24h urine protein quota, the urine red cell count, the serum creatinine, the blood urea nitrogen, Renal morphology and expression of typeⅣcollagen ,and so on.Finally:1,The model group big mouse 24h urine protein quota is significantly increased (P <0.05),the rate of increase of the Shenxuening Jianji group,the Diovan (valsartan) group 24h urine protein quota levels is significantly lower than the rate of the model group (P <0.05),Compared the Shenxuening Jianji group with the Diovan (valsartan) group, there is no statistically significant (P> 0.05).2,The model group big mouse urine red cell count is significantly increased (P <0.05),the rate of increase of the Shenxuening Jianji group urine red cell count levels is significantly lower than the rate of the model group,the Diovan (valsartan) group (P <0.05),Compared the Diovan (valsartan) group with the model group, there is no statistically significant (P> 0.05).3,The model group big mouse serum creatinine,blood urea nitrogen is significantly increased (P <0.05),the rate of increase of the Shenxuening Jianji group,the Diovan (valsartan) group serum creatinine, blood urea nitrogen levels is significantly lower than the rate of the model group (P <0.05),Compared the Shenxuening Jianji group with the Diovan (valsartan) group, there is no statistically significant (P> 0.05).4,Image Analysis:The model group big mouse kidney organization typeⅣCollagen(Col一Ⅳ)average optical density value is significantly increased (P <0.05),the rate of increase of the Shenxuening Jianji group,the Diovan (valsartan) group kidney organization typeⅣCollagen(Col一Ⅳ)average optical density value levels is significantly lower than the rate of the model group (P <0.05),Compared the Shenxuening Jianji group with the Diovan (valsartan) group, there is no statistically significant (P> 0.05).5,Pathology:⑴HE staining results: The model group big mouse glomerular is obviously hypertrophy, glomerular mesangial cells hyperplasia with Mesangial matrix increase, Part of glomerular Segmental sclerosis, ball wall adhesion, Renal tubular epithelial cells degeneration, hypertrophy, hyperplasia, Some tubular atrophy, Renal interstitial have varying degrees of mononuclear inflammatory cell infiltration and fibrosis, the Shenxuening Jianji group,the Diovan (valsartan) group compared to the model group is reduced significantly, and the Shenxuening Jianji group than the Diovan (valsartan) group level light.⑵Immunofluorescence Results: The model group, Shenxuening Jianji group, the Diovan (Valsartan) group big mouse glomerular basement membrane and mesangial area have bright green positive staining deposition, Shenxuening Jianji group, the Diovan (Valsartan) group bright green positive staining deposition less than the model group, and Shenxuening Jianji group bright green positive staining deposition less than the Diovan (Valsartan) group.⑶Immunohistochemical staining results: In the blank control group kidney organization ,typeⅣCollagen(Col一Ⅳ)has the Mild expression in the glomerular basement membrane and mesangial area, the basement membrane proximal tubular epithelial cells, positive staining for the brown granules ,and in the model group, glomerular basement membrane and mesangial area,renal tubular epithelial cells and the basement membrane mesenchymal show a large number of brown particle deposition. Shenxuening Jianji group, the Diovan (Valsartan) group brown granules deposition is significantly reduced compared to the model group,and Shenxuening Jianji group than the Diovan (Valsartan) group level light.Conclusion:1,The Shenxuening Jianji has the function of reducing the IgA nephrosis big mouse 24-hour urine protein and red blood cells, serum creatinine, blood urea nitrogen.2,The Shenxuening Jianji has the function of reducing the primary IgA nephrosis big mouse expression of typeⅣcollagen.3,The Shenxuening Jianji has the function of delaying the IgA nephrosis course development. Its mechanism may be by reducing expression of typeⅣcollagen,to reduce the excessive accumulation of ECM, reduce glomerulosclerosis and tubular - interstitial fibrosis, thereby reduce urine protein,hematuria, and Protect renal function.