| Objective:Cervical cancer is one of the most common malignancies in the worldwide. It is second to breast cancer as the most common malignancies in incidence. This topic seeks to explore a wide range of risk factors of cervical cancer, the relationship of HPV and CIN; focus on folic acid and its metabolic enzyme gene (RFC-1) and MTRR mutation and cancer and the relationship between CIN susceptibility for cervical cancer study provides clues to etiology.Methods:A hospital-based case-control study was conducted in the second hospital of Shanxi Medical University from January 2008 to November 2008.The Research included 400 women The average is divided into five groups:CINⅠ,CINⅡ,CINⅢ, newly diagnosed by pathology of cervical cancer and control of hysteromyoma.The case were frequency-matched with control by age(±3 y). All participants required Shanxi resident,Han people,and people who had cerebrovascular disease,haemolyticus disease,digestive system disease,VitB supplement within 3 months lately and other dysplasias were considered ineligible for study.A standardized questionnaire was used to screen risk factors of cervical cancer,including demographic and lifestyle information,sexual and reproductive history and oral contraceptives using.Blood samples were drawn in before breakfast, serum folate was measured using radiobinding assay,HPV16 was gained by PCR,Genotyping for the MTRR and RFC-1 polymorphism was analyzed by PCR-RFLP. Statistical analysis was conducted by SPSS 11.5 software.Results: (1)Occupation,incomes,education background,many sexual partners,cigarette smoking,menopause,age at first sexual intercourse and parity are related to cervical cancer risk.(2) The positive rate of HPV16 cervical cancer group (60%) was significantly higher (31.25%), with a statistically significant difference (χ2 = 21.52, P = 0.00).(3) HPV16 positive rate of CIN group (46.67%) was significantly higher (31.25%), with a statistically significant difference (χ2 = 5.83, P = 0.02).(4) CINⅠ, CINⅡ, CINⅢ, cervical cancer and control group, five groups of serum folic acid value has been the single factor analysis of variance F = 20.847, P = 0.00, five inter-group differences have statistical significance. With a lower level of serum folate, the incidence of cervical cancer risk may increase.(5) CINⅠ, CINⅡ, CINⅢ, cervical cancer and control group genotype frequencies consistent with Hardy-Weinberg test balance. CINⅠ, CINⅡ, CINⅢand cervical cancer group four MTRR gene 66AA, AG and GG genotype frequencies compared with the control group no significant difference (χ2 = 3.23, P = 0.92). A66G allele distribution between the groups in line (χ2 = 1.80, p = 0.77). MTRR gene 66AA, AG and GG genotypes as from A to G mutation frequency increase in the incidence of cervical cancer risk increased gradually.(6) CINⅠ, CINⅡ, CINⅢ, cervical cancer and control group genotype frequencies consistent with Hardy-Weinberg test balance. The case group RFC-1 gene 80AA, AG and GG genotype frequencies with the control group compared to the gene frequencies, there was no significant difference (χ2 = 6.20, P = 0.63). A80G allele distribution in the two groups are different, there is statistically significant (χ2 = 11.69, p = 0.02). Cervical Cancer Group AA, AG, GG three gene frequencies were 25.00%, 45% and 30%, with the control group, the gene frequency of 41.25%, 42.50% and 16.25% compared to the differences were statistically significant (χ2 = 6.52 , P = 0.04). A80G allele distribution in the two groups have significant difference (χ2 = 7.27, p = 0.00).(7) RFC-1 gene A80G polymorphism and cancer susceptibility results of the analysis showed that carrying AA genotype than those carrying GG genotype the risk of cervical cancer was significantly higher OR = 2.42 (95% CI: 1.01 ~ 5.81 ); and AG genotypes on cervical cancer risk was no significant impact OR = 1.26 (95% CI: 0.56 ~ 2.86)Conclusions: (1)Peasant,smoking,multiparty,earlier age at first intercourse were associated with increased risk of cervical cancer,Menopausal,higher education degree,higher incomes were the protect factors of cervical cancer. (2)HPV16 infection was the etiology associated with the development of cervical cancer. (3) Low serum folate levels are CIN, cervical cancer risk factors, serum folate deficiency to a certain degree of CIN and cervical cancer can increase the risk.(4) serum folate had effect with HPV16 infection in the development of cervical cancer. (5)The mutant type homozygote of RFC-1 A80G was a risk factor of cervical cacer.The women who had RFC-1 80GGgene type were induced to develop to cervical cancer.(6)Although there is no statistic association between MTRR gene A66G polymorphism and cervical cancer,a weak and nonsignificant relationship exits between mutant type homozygote of MTRR gene A66G and cervical cancer. |
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