| Objective: By observing exercise effects on serum Leptin, the hypothalamus MC4R mRNA and BDNF of high-fat diet rats, we wants to expore the appropriate exercise to prevent high-fat diet in rats with obesity and the changes in BDNF mechanism for the prevention and treatment of obesity and provide a theoretical basis.Method: Six-week-old male SD rats with 32, were randomly divided into normal control group, high-fat model group, high-fat 60-min exercise group, high-fat 90-min exercise group. Each group has eight. Exercise group undertake Non-weight-bearing swimming exercise for 10 weeks exercise. After 10 weeks, the detection of lipids (TG, TC, HDL-C, LDL-C), FFA, Leptin, the hypothalamus MC4R mRNA expression , BDNF of hypothalamus is to study the appropriate exercise to prevent high-fat diet in rats with obesity and the changes in BDNF mechanism for the prevention and treatment of obesity .Result: 1. Pre-exercise high-fat diet rats in each group body weight was no significant difference. It wasn't significant that each group of rats feeding high fat diet during the exercise.10 weeks after the exercise, high fat exercise 60-min group, 90-min high fat group exercise control group, a significant reduction in body weight (P <0.01). However, high fat 60-min exercise 90-min exercise group no significant weight. This prompted, 60-min, 90-min of aerobic exercise can be the prevention of obesity in rats fed with high fat diet, exercise and high-fat group reduced body weight but not by feeding through to the effects of exercise. Also described 90-min and 60-min of aerobic exercise aerobic exercise compared to no better results.2. 10 weeks after the swimming exercise, the normal control group, Lee's index was significantly lower than the high-fat control group (p = 0.001 <0.01), hyperlipidemia 60-min exercise group, exercise group 90-min Hyperlipemia Lee's index was significantly lower than high-fat control group (p = 0.012 <0.05, p = 0.017 <0.05). High-fat control group than the normal control group significantly increased body fat (p = 0.039 <0.05), high-fat exercise 60-min group, 90-min exercise group a higher fat fat body fat control group was significantly lower (p = 0.001 <0.01, p = 0.002 <0.01). However, fat body weight ratio in each group no significant difference.3. High-fat rats of model group rats than in normal control group TG, TC, LDL-C significantly increased (p = 0.013 <0.05, p = 0.018 <0.05, p = 0.001 <0.01); Hyperlipemia 60min exercise group, high-fat 90min movement model rats high fat rats TG, TC decreased significantly (p <0.01, p <0.05). 90min exercise fat fat model group, the higher HDL-C significantly increased (p = 0.038 <0.05). Hyperlipidemia model group than normal control group, FFA significantly increased (p <0.01), high-fat 60min exercise group, exercise group fat 90min higher FFA lipid model group decreased significantly (P <0.01). Description of the movement for high-fat diet to correct lipid metabolism disorder in rats4. Hyperlipidemia model group, serum leptin than normal control rats significantly increased (p <0.01); Hyperlipemia 60min exercise group, exercise group 90min fat fat rat model of high serum leptin decreased significantly ( p <0.01). Exercise group and two groups of high-fat and serum leptin compared to normal control rats was no significant difference in that high-fat meal can cause levels of serum leptin levels significantly increased, while the movement to correct high-fat diet rats elevated serum leptin, and reduced to normal levels, to improve the high-fat diet rats, "leptin resistance."5. After 10 weeks of swimming, high-fat rats of model group than normal control rats MC4R mRNA significantly decreased (P <0.01), high-fat 60min exercise group, exercise group 90min fat fat rat model of high MC4R mRNA in rats significantly increased (P <0.01). 60min movement but high-fat group, high-fat rats 90min movement MC4R mRNA expression was no significant difference. 6. High-fat rats of model group than normal control group, a significant reduction in BDNF in rats (p = 0.021 <0.05), high-fat 60min exercise group, high-fat rats 90min higher lipid movement in model group rats significantly increased (p = 0.018 <0.05, p = 0.026 <0.05). High-fat exercise group compared with normal control group without statistical significance. That high-fat meal can cause the hypothalamus of rats a significant reduction in BDNF, and the movement to correct high-fat diet reduced BDNF rats and rose to normal levels.Conclusion: 1. Appropriate long-term exercise can prevent obesity in rats fed with high fat diet, and found that 90 minutes of exercise and 60 minutes of exercise on body weight, no significant difference. 60 minutes of movement that can play a role in the prevention of obesity.2. High-fat exercise fat rat model of high serum Leptin significantly reduced, suggesting that appropriate long-term campaign to improve high-fat diet rats, "leptin resistance" so that the hypothalamus of leptin play a role more fully. High-fat rats a high fat movement model rats BDNF content in the hypothalamus, MC4R mRNA expression increased significantly. Its possible mechanisms are: a long-term campaign to make the body suitable for a large number of energy consumption, increased decomposition of adipose tissue, resulting in decreased fat reserves, adipose tissue, "perceived" to these changes and reduce the Leptin secretion and improve the sensitivity of exogenous leptin and the brain / blood leptin than the increase in obesity in order to improve the blood-brain barrier transport barriers, increased leptin into the brain. Leptin and leptin receptor binding, induction of ARC expression of POMC toα-MSH secretion axon, with activation of the hypothalamic neurons of the MC4R receptor, MC4R stimulate BDNF release, BDNF acting on a sense of satiety and Secretary motor neuron , thereby reducing food intake, to stimulate the movement, so that increased energy consumption. |
PDF Full Text Request