| Objective:This issue is at my country house presided over "85" key issues - "broken blood removing extravasted blood, flows swiftly the hot inducing resuscitation, the phlegm reduction to straighten out the treatment acute cerebral hemorrhage's experiment and the clinical research ", based on each group of rats Determination of serum S100 protein, analysis, to explore traditional Chinese medicine in treating acute cerebral hemorrhage the scope of the best amount of bleeding.Acute intracerebral hemorrhage has been a threat to human health. It is the main killer of poor prognosis. At my country house presided over "85" key issues, while Chinese medicine has been initially established in the treatment of acute cerebral hemorrhage critical bleeding volume <50ml, but the amount of bleeding between 1 ~ 50ml from a wider range of clinical studies concluded that: effective, significant differences between the efficiency of larger. Therefore, looking for the largest medical conservative treatment of critical bleeding volume and the specific diagnosis and treatment of related standards, corresponding to the depth objective indicators become eager to solve.In this study, experimental serum S100 (neural glial cell line-specific marker protein), as the research object, in this theory, based on the use of enzyme-linked immunosorbent assay, by observing in experimental acute cerebral hemorrhage in rats of different amount of bleeding Serum S100 protein levels in a further study of Chinese medicine to acute cerebral hemorrhage (bleeding volume <50ml and the exception of hypothalamus, brain stem, cerebellar hemorrhage in the case of) the best medical conservative treatment threshold, with a view to looking for traditional Chinese medicine in treating acute cerebral hemorrhage best indication for the diagnosis and treatment standards and clinical indicators for specific detection of selection.Method: Health, adult wistar rats 72, male, weight about 300g. For sham-operated group served as controls, eight rats; Chinese control group and cerebral hemorrhage model group, according to the different amount of bleeding was divided into 27ul, 40ul, 53ul, 67ul four groups of eight rats. Model group and sham-operated group after model 1.5ml saline gavage and intraperitoneal injection, 1 times/day; treatment group after delivery model, the daily capsule 12.5mg/100g God Xingnaojing Kin weight, soluble in water for injection 1.5ml gavage, 1 times / day, Qingkailing 1.3ml, dissolved in 1.5ml water for injection by intraperitoneal injection , 1 times / day. After above animal technique, continues to give the medicine 3 days. 3 days later, weighing, use of 1% sodium pentobarbital anesthesia animals, abdominal aortic blood about 4ml, static at room temperature 30-60min, 4000 rev / min centrifugation of the centrifuge 4 minutes. Take the supernatant, -80℃refrigerator to preserve. Determination blood serum S100 protein density.Result: Rats 3 days after hemorrhage, model group and treatment group NDS was significantly higher than sham operation group (P <0.01), but the NDS treatment group was significantly lower than model group (P <0.05), Prompted the intervention of Chinese medicine, the cerebral hemorrhage model rats of various amount of bleeding is the cause of neurological deficit has been significantly improved. Rats 3 days after hemorrhage, inter-group comparison, 27ul,40ul blood serum levels of S100 protein value of the model group was significantly higher than treatment group and sham operation group (p <0.01), treatment group with little difference between sham-operated group (p> 0.05); 53ul,67ul blood serum levels of S100 protein value of the model group was significantly higher than sham operation group (p <0.01), treatment group compared with the sham-operated group, although there is significant difference values (p <0.01), but with the model group ratio, S100 protein levels still decreased significantly. Group comparison: Model group, group comparison: Serum S100 protein levels in 27ul and 40ul,53ul,67ul are statistically significant comparison (p <0.01), 40ul,53ul and 67ul comparison between the no significant difference (p> 0.05); treatment group: Serum S100 protein levels in 27ul and 40ul was no significant difference (p> 0.05), 53ul and 67ul no significant difference (p> 0.05), 27ul,40ul with 53ul,67ul comparison were significantly different ( p <0.01).Conclusion: Cerebral hemorrhage in stress conditions, Chinese medicine treatment on the nerve cell membrane and blood-brain barrier of protection has a strong effect; Chinese medicine treatment of acute cerebral hemorrhage best possible for the critical amount of bleeding 30ml (excluded from the hypothalamus, brain stem, cerebellar hemorrhage); Serum S100 cerebral hemorrhage irreversible brain injury to reach the level of Chinese medicine treatment of initial value of effective community 6.3ng/ml-8.16ng/ml. |
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