Effect Of Resveratrol On Mmp-9 And Timp-1 Expression In Ischemic Brain Injury In Neonatal Rats With Hypoxic And Research

Objective To explore the regular expression of MatrixMetalloproteinase -9 (MMP-9),TissueInhibitors of MatrixMetalloproteinase -1(TIMP-l) as well as the disruption of blood brain barrier in neonatal hypoxic-ischemic brain damage(HIBD) rats. To study the effect of resveratrol (RES) on cerebral edema as well as the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1(TIMP-1). Methods Totally 119 7-day-old wistar rats were randomly divided into 3 groups:Sham-operated group (n=36),HIBD group (n=38),HIBD+RES group (n=38). The time (6 h,24 h,48 h,72 h,5d,7d) was established in these three groups. The expression of MMP-9 and TIMP-1 was investigated with immunohistochemistry and the water content was calculated with dry and wet weight method after 6 h,1d,2d,3d,5d and 7 d of reperfusion . Results 1.MMP-9 positive expression was rarely detected in Sham-operated group,while it increased in HIBD 6h,increased markedly at 24h,reached peak levels at 48h ,after this,the level of-MMP-9 began to decrease up to 7d. TIMP-1 positive expression was rarely detected in Sham-operated group , increased markedly in HIBD 6h, reached maximal levels at 24h,compared with 24h the level of TIMP-1 began to decreas at 48h and the decrease continued up to 7d group.The water content increased at 6h,then it were obviously increased at 24h,reached peak level at 48h,there was significance difference between sham group and HIBD group (P<0.01). 2. In resveratrol group compared with HIBDgroup,The water content,the expression of MMP-9 and TIMP-1 were significantly decreased after 1d,2d,3d and 5d of reperfusion.There was significance difference. (P<0.01). Conclusion 1.The expressions of MMP-9 and TIMP-1 were depending on different stage of reperfusion. The study suggestinged that MMP-9 may play an important role in early HIBD, and it is one of the factors of brain edema forming, and can result in the form of VBE.2. RES can suppresses the expression of MMP-9 and maintain integrity of BBBso that lighten the level of VBE . That may be one of the mechanisms of neuroproteetive effect of RES .