Low Molecular Weight Heparin On The Rat Model Of Chronic Obstructive Pulmonary Disease Cells Adhesion Molecule-1, Monocyte Chemoattractant Protein-1 Expression

Objective To observe the effect of low molecular weight heparin(LMWH) on expression of intercellular adhesion molecular-1(ICAM-1) and monocyte chemoattractant protein-1(MCP-1) in lung tissue of rats with chronic obstructive pulmonary disease(COPD), then demonstrate the intervention mechanism for chronic obstructive pulmonary disease of low molecular weight heparin.Methods Thirty Wistar rats were randomly divided into three groups,with 10 in each:â‘ normal control group:raised the rats in normal condition for forty days without any interferende;â‘¡COPD model group:The COPD model was established by intratracheal instillation of lipopolysaccharide and exposed to cigarette smoking;â‘¢LMWH treated group:model methods were the same as above, LMWH 150U/kg was given by hypodermic injection,daily, Pathologic changes of the lung tissue,blood gas and the total and differential cell counts of bronchoalveolar lavage fluid were determined.The expression of ICAM-1 and MCP-1 was observed by immunohistochemical method..Results The pathological changes in COPD model group was coincident with that in humans.â‘ Compared with normal control group, The expression of ICAM-1,MCP-1 of lung tissue of the COPD group significantly increased (P<0.05), and ICAM-1 was positively correlated with the leukocyte numbers and neutrophils in BALF in the model group(r=0.82,t=4.00,P<0.01),MCP-1 was positively correlated with the macrophage in BALF in the model group(r=0.81, t=3.96,P<0.01);â‘¡Compared with COPD model group,pathological changes of the lung tissue in LMWH treated group decreased.The total white blood cells and the percentages of neutrophils in BALF in LMWH treated group decreased (P<0.01).The expression of ICAM-1 and MCP-1 in LMWH treated group decreased (P<0.05,0.01)Conclusion:ICAM-1 and MCP-1 may be involved in the process of airway inflammation in COPD.LMWH may decrease the expression of ICAM-1 and MCP-1 to relievate the inflammatory reaction.