| Background and Objectives: chronic obstructive pulmonary disease (COPD) is a global health problem with increasing morbidity and mortality, but its mechanisms are not very clear. Important characteristics of COPD is incompletely reversible airflow limitation, airway inflammation and remodeling is pathological fundament of COPD patients' airflow obstructive and irreversible airflow limitation,they play important roles in the generation, development and prognosis of COPD, especially, airway remodeling is a determinant factor of COPD patients' irreversible airflow obstructive and is a importment reason that COPD can't recovery completely. Some research have finded that the cytokines and chemokines play a very importment role in the pathogenesis of COPD in recent years, monocyte chemoattractant protein-1 (MCP-1) is a number of C-C chemokines family, It is expressed in monocytes/macrophages,fibroblasts,B cell, vascular endothelial cells, vascular smooth muscle cells, and several cancer cell lines . its can attract and activate many inflammatory cells, such as monocytes/macrophages, lymphocyte, mast cell, neutrophils and NK cell, in addition its can induce collagen and transforming growth factor-teba expression by fibroblasts. transforming growth factor-betal (TGF-β1) can promote the proliferation and migration of airway smooth muscle cells proliferation of fibroblast, induces fibroblast convert to myofibroblast, also stimulates the production of extracelluar matrix (ECM) components,but inhibits degradation of ECM. At presentthere are some researchs about MCP-1 and TGF- P 1 in induced sputum, bronchoalveolar lavage fluid (BALF) and airway epithelium, but the serum levels and significance of MCP-1>TGF- 0 -1 in Patients with COPD remains unreported, the relationship between MCP-1 and TGF- P~l is not defined. To elucidate the relationship between MCP-1 and TGF- 3-1 and their pathophysiologic role and significance in COPD, we measured serum levels both in acute exacerbation and remission stages in 29 cases of COPD and analyse its correlation with FEVl%pre and FEV1/FVC .which reflects the obstructive degree of COPD, so that we can find a new treatment for COPD.Subject and Method: COPD group comprising 29 Patients (20 males and 9 females aged 45 to 75 years; mean zfcSdage, 61 + 8. 4years) was classified into groups (acute exacerbation and remission stage) according to their clinical presentation. 29 gender-and age-matched healthy subjects served as a normal control group. The Serum Levels of MCP-1 > TGF-0-1 were detected by enzyme linked immunosorbent assay (ELISA). TheFEVl%pre and FEV1/FVC were measured with lung function apparatus.Results: (1) The serum levels of MCP-1 in the acute exacerbation and remission stages of COPD group [ (30.96+13.43) pg/ml, ( 22. 73 + 11. 63) pg/ml] were both higher than those in the normal control group [(13.77 + 8. 69)pg/ml, p<0. 01,/K0.05], and the serum levels of MCP-1 in the acute exacerbation stage was higher than those in the remission stage (p<0. 01). (2) The serum levels of TGF- 3 -1 in the acute exacerbation and remission stages of COPD group [(130. 93+33. 29)pg/ml, (104. 60+31. 43)pg/ml] were both higher than those in the normal control group[(84.45 +21.38)pg/ml,/rt0.01,/X0.05],and the serum levels of TGF-0-1 in the acute exacerbation stage was higher than those in the remission stage(/K0. 01). (3) In COPD group, the serum levels of MCP-1 negatively correlated with FEVl%pre(r=-0.895, p<0. 01; r=-0.898^<0. 01) and FEVl/FVC(r=-O.8O2, p<0. 01;r=~0. 777, ^<0.01),in the acute exacerbation and remission stages. (4) InCOPD group, the serum levels of TGF- P -1 negatively correlated with FEVl%pre ( r=-0.809, p<0.01;r=-0.798, p<0.01 ) and FEVl/FVC(r=-0.701, p<0. 01 ;r=-0. 677, p<0. 01) ,in the acute exacerbation and remission stages. (5) In COPD group, the serum levels of MCP-1 positively correlated with TGF- P -1 (r=0.847,/KO. 01; r=0. 759, p<0.01) , in the acute exacerbation and remission stages.Conclusions: (1) The serum levels of MCP-1 in the acute exacerbation and remission stages in patients with COPD group were both higher than those in the normal control group, especially in the former stage. In COPD group, the serum levels of MCP-1 negatively correlated with FEVl%pre and FEV1/FVC both in the acute exacerbation and remission stages.(2) The serum levels of TGF- P -1 in the acute exacerbation and remission stages with COPD group were both higher than those in the normal control group, especially in the acute exacerbation. In COPD group, the serum levels of TGF- P -1 negatively correlated with FEVl%pre and FEV1/FVC both in two stages.The encrease in serum levels of MCP-1 and TGF-P -1 is a close correlation with airway inflammation and airway remodeling of patients with COPD. These finding suggests that MCP-1 and TGF- P -1 participate in the development of COPD and play an important role in the pathophysiology of COPD. Blocking its signal transduction or inhabiting biological activity may become a new therapy for COPD.
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