Celecoxib Reck Gene In Gastric Cancer Cell Mgc-803, Mmp-2, Mmp-9 Expression,

Objective: To study the role of non-steroidal anti-inflammatory drug (NSAID) celecoxib in downregulation the expression of RECK gene in MGC-803 cells, and its relationship with MMP-2 ,MMP-9 and the anti-tumor mechanisms of celecoxib were explored.Methods: The gastric cancer cell MGC-803 was starved in serum-free mediumed for 24h to synchronize. The cells were treated with different concentrations of celecoxib (25,50,100μg/L) in different time(12h,24h,48h), and the following methods were used :(1) The MGC-803 cell proliferation observed by MTT.(2) The expressions of MMP-9, MMP-2 and RECK mRNA were detected by RT-PCR.(3) The expressions of MMP-9, MMP-2 and RECK protein were detected by Western-blot.Results: (1) MTT assay showed gastric cancer MGC-803 cell proliferation was inhibited by celecoxib, along with concentration of celecoxib increased, the inhibitory effection was not significant at 12h but in concentration-dependent (25-100μg / L) after 24h and 48h. The inhibitory role was time-dependent(12-48h) at 25,50,100μg/L concentration. Cell proliferation was mostly inhibited at 100μg / L after 48h. RT-PCR assay showed that the expressions of RECK, MMP-2 and MMP-9 were not significant (P> 0.05) while concentration of celecoxib increased. After 12 hours, Celecoxib increased RECK mRNA expression in concentration (25-100μg / L) and time-dependent (24-48h) manners, but decreased the expressions of MMP-2 and MMP-9.(3) Western-blot showed: Along with celecoxib concentration increased at 12h, the expressions of RECK, MMP-2 and MMP-9 were not change significantly compared with controls(P>0.05). After 12h, celecoxib increased the expression of RECK gene in concentration-dependent (25-100μg / L) and time-dependent (24-48h) manners, but decreased the expressions of MMP-2 and MMP-9 protein.Conclusions: (1) Celecoxib can inhibit the proliferation and metastasis of the gastric cancer MGC-803 cells.(2) The mechanism of celecoxib anti-tumor may via upregulation the expression of RECK gene, and downregulation the expressions of MMP-2 and MMP-9 to inhibit gastric cancer MGC-803 proliferation and metastasis.