Effects Of Proliferation And Apoptosis And COX-2Expression By Celecoxib With Curcumin In SGC-7901Human Gastric Cancer Cell

Objective: To investigate the effects of curcumin with celecoxib,a selective COX-2inhibitor, on cell proliferation and apoptos,COX-2Expression in SGC-7901human gastriccancer cell line and to explore its anti-neoplasm mechanism.Methods：MTT assay was used to determine cell proliferation after incubation indifferent concentrations of curcumin with celecoxib，The effect of curcumin with celecoxibon cell cycle changes and apoptosis of cells was studied by flow cytometry(FCM)，themRNA expressions of COX-2were analyzed by RT-PCR; Western Blot was used to detectCOX-2protein expression.Results:(1) MTT showed：curcumin with celecoxib on gastric cancer SGC-7901cells was inhibited, the inhibition increased with increasing doses of the drug, the choice ofno significant effects on cells of low doses of curcumin with celecoxib combined withsignificantly enhanced when compared with the inhibitory effect of curcumin。There weregreat statistical signification between medicated group each other (P<0.05).(2) Flowcytometry results suggest that the combination group G0/G1phase cells in comparisonwith curcumin or celecoxib group was significantly increased, S phase and G2/M phasewas significantly reduced (P<0.05).(3) RT-PCR and Western Blot results showed thatcurcumin or celecoxib and the combination group,COX-2expression was significantlyreduced compared with the control group significantly (P <0.05), combination groupcompared with single drug group Statistically significant (P<0.05)。Conclusion： Curcumin could inhibit the growth of human SGC-7901Gastricadenocarcinoma cells, which enhance desensitivity of curcumin on SGC-7901cells.Thecureumin and celecoxib can all induce human SGC-7901gastric Adenocarcinoma cellsapoptosis. When the two drugs combined，the inductive effect for cell apoptosis becamemore obvious. which may provide a theoretical basis for their use in chemoprevention of gastric cancel．...