Effects Of Apoptosis And COX-2, Bcl-2 And Bax Expression By Celecoxib With Fluorouracil In SGC-7901 Human Gastric Cancer Cell

Objective:To investigat the effects of celecoxib,a selective COX-2 inhibitor,with 5-Fu on cell proliferation,COX-2,Bcl-2 and Bax Expression and apoptos is in SGC-7901 human gastric cancer cell line and to explore its anti-neoplasm mechanism.Methods:MTT assay was used to determine cell proliferation after incubation in different concentrations of celecoxib with 5-Fu ,The effect of celecoxib with 5-Fu on cell cycle changes and apoptosis of cells was studied by flow cytometry(FCM),The immunohistochemistry measured the expression of COX-2,Bcl-2 and Bax proteins .Results:MTT showed :Celecoxib (celecoxib) and 5-Fu on gastric cancer SGC-7901 cells was inhibited, the inhibition increased with increasing doses of the drug, the choice of no significant effects on cells of low doses of celecoxib (celecoxib ) And 5-Fu combined with significantly enhanced when compared with the inhibitory effect of 5-Fu and the combination group were alone with the same dose of 5-Fu group, the differences were statistically significant (P <0.05),no significant effects on cells of low doses of celecoxib and 5-Fu combined with significantly enhanced when compared with the inhibitory effect of 5-Fu. Flow cytometry results suggest that the combination group G0/G1 phase cells in comparison with celecoxib (celecoxib) or 5-Fu group was significantly increased, S phase and G2 / M phase was significantly reduced. Immunocytochemistry results showed that celecoxib and 5-Fu and the combination group, COX-2, Bcl-2 expression was significantly reduced compared with the control group significantly (P <0.05), combination group compared with single drug group Statistically significant (P <0.05); celecoxib (celecoxib) the role of Bax expression in cells increased significantly compared with the control group, but 5-Fu alone was no significant expression (P> 0.05), the Joint Group Bax Expression increased significantly compared with single-agent group.Conclusions: The anticancer activity of Low toxicity and low-dose celecoxib is weak, but combination with the chemotherapy drug 5-Fu can greatly enhance the anticancer effect of 5-Fu and enhance the drug sensitivity of 5-Fu;celecoxib with 5-Fu induced apoptosis of human gastric cancer cells, and it may have a relationship with inhibition of COX-2, Bcl-2 protein expression and induction of Bax protein expression, leading to intracellular ratio of Bcl-2 and Bax change.