| Purpose: To study the pharmacodynamical effect of Ginkgo Biloba Extract (GBE) as an alpha-glucosidase inhibitors (α-GI).Method: Via the experiment of carbohydrate loading on normal rats and diabetic model rats, I have observed the action of GBE to lower the concentration of blood glucose after meals; I confirm the inhibitive effect of GBE by using the inhibitive experiments out of body on the alpha-glucosidase which was extracted from the small intestine and everted intestinal segment method ex vivo in different situations and sugars. Further more, I target the GBE on amylase and maltase, to survey the objective enzyme of inhibition during the process of starch digestion. I prepared different concentrates of GBE to observe the dose-effect relationship and the IC50 of sucrase and amylase. The double-reciprocal inhibitive curves were drawn to determine the mechanism of GBE inhibition.Results: GBE has evident effect on the concentration of blood glucose of diabetic model rats after meals, but little effect on normal rats. The out-of-body and ex-vivo experiments show GBE the action of pharmacodynamics as anα-GI further. GBE is able to inhibit the enzymatic hydrolization to sucrose, starch and maltose. The IC50 of sucrase is 0.7586mg/ml and IC50 of amylase is 0.9101mg/ml. The mechanism of GBE inhibition is uncompetitive inhibition.Conclusion: GBE can reduce the concentration of blood glucose of diabetic model rats after giving sucrose or starch evidently, however, it has little effect on the absorption of glucose. A series of out-of-body experiment also indicated that GBE has superior inhibition onα-glucosidase. |
PDF Full Text Request