| Objectives To observe the improvement of glucose metabolism in T2 DM GK rats and study the inhibitory effect on Alpha-glucosidase of Shizidaiping Formula,it's 10 herbs and10 main ingredients from 10 herbs,try to explain the mechanism of hypoglycemic effect,and provide theoretical basis for the development of new Alpha-glucosidase inhibitors in further.Methods 1 In vivo experiment: Select 45 SPF male GK rats,T2 DM model was established by feeding high fat feed for 4 weeks after adaptive feeding 1 week, detect 2h plasma glucose(PG2h), PG2h>11.1 mmol·L-1 was considered succeed T2 DM model..And the T2 DM rats were randomly divided into model group, acarbose group, Shizidaiping Formula groups with high, medium, low dose respectively(4g/kg, 8g/kg, 16g/kg)and normal group. The normal group was feeding by ordinary forage.Oral glucose tolerance test(OGTT)was detected for the first time before dosage, dosing 4 weekendand at the ends of the 8th week,,calculate the area under the curve. Hb Alc was detected at the end of the 8th week; 2 In vitro experiment: Using sucrose as a substrate, create a vitro filter model of Alpha-glucosidase activity inhibitory, then Observe the influence of Alpha-glucosidase enzymes on each drug group, acarbose used as the positive control group. Different concentrations of water-extraction preparation of Shizidaiping Formula, it's ten herbs and10 ingredients were used to drug intervention. Glucose oxidase were determined in Shizidaiping Formula, it's 10 herbs and 10 main ingredients of Alpha-glucosidase inhibitory effect, the results were reported with absorbance units. Calculate the inhibitory ratio and IC50 values for each group finally.Results 1 In vivo experiment showed that: Shizidaiping Formula can reduce the levels of FBG and PG2 h in GK T2 DM rats. Compared with the model group, there were significant differences in each dose group(P< 0.05, P<0.01), and the effect was dose dependent. Ten offspring can reduce the level of GK in T2 DM Hb Alc rats, each dose group has significant difference compared with model group(P<0.05). After 4 weeks of treatment, OGTT curves of different doses of ten square rats area compared with the model group, there was no significant difference(P>0.05); after 8 weeks of treatment, OGTT curves of different doses of ten square rats area and the model group compared with still no significant diffeence(P>0.05), but the OGTT curve area and before compared were decreased to some extent, was dose dependent; 2 In vitro experiment showed that: Each concentrations of water-extraction preparation of Shizidaiping Formula, it's ten herbs and 10 ingredients could inhibit the activity of Alpha-glucosidase. Shizidaiping Formula, Tusizi, Chongyuzi,Wuweizi, Laifuzi, Suzi, acarbose have higher inhibiting rates: 60.20%, 55.32%, 50.67%,54.01%, 53.13%, 58.49%, 87.69% at the Concentration of 125mgl/m L. At the Concentration of 40mmol/L, Rosmarinicacid, Hyperin, Sinapine, thiocyanate have the highest inhibiting rates 93.52%, 87.10% and 78.11%. while acarbose(25.82mg/m L) was90.89%. Shizidaiping Formula, Tusizi, Chongyuzi, Wuweizi, Laifuzi, Suzi, Rosmarinic acid, Hyperoside, Sinapine thiocyanate and Acarbose on Alpha-glucosidase inhibitory effect of the IC50 value were 16.057mg/m L, 44.401mg/m L, 89.608mg/m L, 72.146mg/m L,82.139mg/m L, 21.549mg/m L, 0.412mg/m L, 0.605mg/m L, 0.806mg/m L and 0.376mg/m L.Conclusions 1 Shizidaiping Formula can reduce GK rats FBG, PG2 H and HbAlc level;Enhance the body's glucose tolerance; Promote the metabolism of sugar and ameliorate the state of insulin resistance; 2 Each concentration of water-extraction preparation of Shizidaiping Formula, it ' s ten herbs and 10 ingredients could inhibit the activity of Alpha-glucosidase. Shizidaiping Formula, Tusizi, Chongyuzi, Wuweizi, Laifuzi, Suzi and Rosmarinicacid, Hyperin, Sinapine thiocyanate have the highest inhibiting rates. |
PDF Full Text Request