Fabrication Of Modified Electrodes And The Electrical Separation Of Biological Molecules

The chemically modified electrodes(CMEs) is a new reaearch field, which was began to develop in the mid-70s of the 20th century, it is one of the most important of electrochemicals and electroanalytic chemicals. Now, CMEs have been founded numerous important applications in biological science, environmental science, food science, material science and analytical science and so on. The CMEs not only can according to the wishes and needs of deifferent people to modified electrode surface with different material to make different types of modified electrode, but also the modified electrode has a different role to the material of the solution, so the modified electrode has good selectivity. Based on these advantages of the above, in this paper we were prepared modified electrodes, and its application in separation were studied.1.Simultaneous determination for dopamine, ascorbic acid and uric acid at the Poly- poly (4-aminobenzene sulfonic acid) modified electrodeA modified glassy carbon electrode (GCE) with poly (4-aminobenzene sulfonic acid) (ABSA) has been fabricated through an electrochemical oxidation procedure and was used to electrochemically detect dopamine (DA), ascorbic acid (AA), uric acid (UA)and their ternary mixture by cyclic voltammetry (CV) and linear sweep voltammetry (LSV) method and this modified electrode was studied. The modified electrode displayed excellent electrochemical catalytic activities toward DA, AA and UA. For the ternary mixture containing AA, DA and UA, the three compound can well separated from each other with a potential seperation of 265 mV, 146 mV and 411 mV in LSV between AA and DA, DA and UA, AA and UA, respectively. The seperations are large enough to determine AA, DA and UA individually and simultaneously. The first time achieved the seperation of AA, DA and UA at this modified electrode, and the modified electrode behaved with good repeatability and stability.2.Simultaneous determination for dopamine, ascorbic acid and uric acid at the modified glassy electrode with XC―72CB―chitosan.A modified glassy carbon electrode (GCE) with XC-72 Carbon Black has been fabricated and was used to electrochemically detect dopamine (DA), ascorbic acid (AA), uric acid (UA)and their ternary mixture by cyclic voltammetry (CV) and linear sweep voltammetry (LSV) method and this modified electrode was studied. The modified electrode displayed excellent electrochemical catalytic activities toward DA, AA and UA. For the ternary mixture containing AA, DA and UA, the three compound can well separated from each other with a potential seperation of 254 mV, 140 mV and 394 mV in LSV between AA and DA, DA and UA, AA and UA, respectively. The seperations are large enough to determine AA, DA and UA individually and simultaneously. In this experiment, the modified electrode is facile to fabricated and behaved with good repeatability and stability, the first time was achieved on the determine of AA, DA and UA at the same time in the modified electrode.3.Simultaneous determination for sulfamethoxazole and trimethoprim at the modified glassy electrode with XC―72CB―Nafion.A modified glassy carbon electrode (GCE) with XC-72 Carbon Black has been fabricated and was used to electrochemically detect sulfamethoxazole (SMZ), trimethoprim (TMP) and their mixture by cyclic voltammetry (CV) and differential pluse voltammetry (DPV) method and this modified electrode was studied. The experimental results showed that the electrochemical oxidation of SMZ and TMP was sluggish on GCE, and the modified electrode displayed excellent electrochemical catalytic activities toward SMZ and TMP. For the mixture containing TMP and SMZ, the compound can well separated from each other with a potential seperation of 250 mV. The seperations are large enough to determine TMP and SMZ individually and simultaneously. This C-Nafion/GCE could be applied in SMZ and TMP electrochemical determination with satisfied results. In this study, the first time completed the simultaneous determination of TMP and SMZ in a facile fabricate modified electrode, and the result are satisfactory. At the same time the proposed method can be applied to the determination of SMZ and TMP in tablet samples.