Studies On The Extraction, Purification And Semi-synthesis Of Vinblastine

Catharanthus roseus (L.) G. Don is one of the important medicinal plants of Catharanthus in Apocynaceae.Many important indole alkaloids were found in Catha-ranthus roseus,in which Vinblastine(VLB) and Vincristine(VCR) were widely used for anti-cancers in clinic,and Catharanthine(CTR) and Vindoline(VDL) were important materials for semi-synthesis of Catharanthus alkaloids.In this article,In order to im-provethe utilization of Catharanthus roseus's.the process of extraction,isolation and semi-synthesis of VLB were studied,and a mode for comprehensive utilization of Ca-tharanthus roseus was established.1. Establish a extraction method for Catharanthus roseus in industry,to obtain VLB Catharanthine(CTR) and Vindoline(VDL).The extraction method use four times 80% ethanol-water(v/v) and extraction three times.2. Vinblastine were separated into mono-indole alkaloids and dimeric-indole al-kaloids by pH gradient extraction method, and VLB Catharanthine(CTR) and Vindo-line(VDL) were obtained by chromatography method.3. Synthesize Dichromic acid with VLB to obtain Vincristine(VCR) by one-step reaction, and the 99% purity of Vincristine(VCR) was obtained by chromatography method..4. Couple Catharanthine(CTR) with Vindoline(VDL)'s to obtain Anhydrovinblas-tine(AVLB), then go through bromination and Ring-Closure Reaction-, further go through chromatography separation, then 99% purity of Vinorelbine(VNR) was ob-tained.