| Compared with aerobic biological treatment technology,anaerobic biological treatment technique with a high load factor, low power and low sludge yield advantages. This paper complex immobilized microorganisms to explore the effects of ammonia nitrogen.The sludge of coking wastewater through isolated process of the collection, culture, purification, and it is successed to get five strains, named NB-1, NK-1, NK-2, NS-1, NS-2. And wastewater in the experimental environment can survive, and good environmental adaptability; and five kinds of single strain 1:1 complex, get 10 complex microorganisms, the experimental determination of the degradation efficiency of ammonia water. The results show that, NK-1 and NS-1 the most obvious advantage of complex bacterium, named NKS-1, occurred between niche separation and form mutually beneficial symbiotic relationship; and the best process conditions are:pH 7.5, temparature of anaerobic incubator 35℃. NKS-1 seed liquid 10 ml was inoculated into 50 ml sterilized domestic sewage in 150 ml conical flask under the mentioned best process conditions, and after36h NH3-N removal efficiency by NKS-1 is 54.33%.Bacteria was immobilized on the composite. Identified the compound bacteria NKS-1 immobilization conditions, determine the optimal value of each factor, analysis of variance by orthogonal experiment and determine the optimal conditions for immobilized Experimental results illustrate the best conditions:Sodium alginate 4%, volume concentration of CaCl2 2%, mass fraction of Package Inocula 3gL-1.Immobilized complex bacteria NKS-1 tower biofilm with sludge in the AF, waste water treatment with ammonia, in the guarantee of hydraulic loading, hydraulic retention time (HRT) 4h conditions; with the functional ceramic, general sludge Biofilm, biofilm for 12 days, the ammonia nitrogen removal rate was 65.5%;with the functional ceramic, biofilm by composite immobilized bacteria NKS-1, linked to film for 7 days, the ammonia nitrogen removal rate was 89.7%. |
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