| Chemiluminescence analysis is based on the intensity of light radiation generated in the chemiluminesce reaction. For a long time, chemiluminescence analysis has been confined to the molecular, atomic fields. Nano-materials showed many strange physical and chemical properties as their special Kubo effect, small size effect, surface effect and quantum tunneling. Recently, more and more people focus on the application of nano-materials.In this paper,we added Ag nanoparticles to luminol chemiluminescence system, and found it has a significant characteristic of sensitizing effect on the intensity of luminol chemiluminescence. On the basis of this result, we carried out the detection of some drugs selectively. The main reaserch contents include as follows:1. It was found that AgNPs could enhance the chemiluminescence intensity of luminol-potassium ferricyanide system. On the basis of enhancement effect, a flow injection method was developed for the determination of Captopril, and a possible mechanism was also discussed. In the optimum conditions, the linear range was 1.0×10-8 5.0×10-5g/L and the detection limit was 2×10-9g/L. The relative standard deviation was 2.6 % for 1.0×10-6g/L Captopril (n = 11). The method has been successfully applied to the determination of Captopril in urine and drugs, The recovery was between 96.0%102.5% and 97.2%～101.1%.2. It was found that AgNPs could enhance the chemiluminescence intensity of luminol-hydrogen peroxide system. On the basis of enhancement effect, a flow injection method was developed for the determination of Dopamine Hydrochloride. In the optimum conditions, the linear range was 1.0×10-4 1.0×10-6g/L and the detection limit was 8.4×10-7g/L. The relative standard deviation was 1.4 % for 5.0×10-5 g/L Dopamine Hydrochloride (n = 11). The method has been successfully applied to the determination of Dopamine Hydrochloride in samples and the recovery was between 98.5 % 103.8%.3. Based on the sensitizing effect of AgNPs on the luminol - potassium ferricyanide CL system, a flow injection method was developed for the determination of trace resorcinol. The concentration of luminol, potassium ferricyanide, sodium hydroxide and AgNPs on CL reaction was investigated respectively. In the optimum conditions, the linear range was 1.0×10-71.0×10-5g/L and the detection limit was 2×10-8g/L. The relative standard deviation was 3.6% for 1.0×10-6 g/L resorcinol(n = 11). The method has been successfully applied to the determination of trace resorcinol in samples and the recovery was between 97.5%101.0%. |
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