The Design Of Chemical Probe For Heavy Metal Ions And Inorganic Anions And Their Recognition Mechanism Study

Cations and anions play an important role in our lives, especially, they are very important in the biochemical processes. Many scientists are interest in detection and identification of cations, for example chemists, clinical biochemists, biologists. Cations influence on our lives greatly, each of metal ions is beneficial for the human health and the nature, while is harm to us. As well as the anion, they also play a very important role in the biological and chemical processes. The anion is ubiquitous in organisms, and they carry the genetic information, the enzyme substrate and composite factors are anions. So many scientists try their best to design and synthesize the chemical probes to recognize the anions and cations.The chemical probes play an important role in the recognition and detection for cations and anions. Rhodamine B dyes is consider as one of the most important chemical probe in recent years, Rhodamine B dyes has been widely used as early as 20 years in the last century. Rhodamine B is good at photophysical properties, its extinction coefficient and fluorescence quantum yield is higher, and there is good light stability, which is the reason that attracted the great interest for scholars.According to this thesis, we design and synthesize a compound of Rhodamine B, we found the detection performance of the derivative is sensitive and selective. Rhodamine-based sensor is capable of detecting both Cu2+ and VO2+ using two different mode. Pyrocatechol violet is a phenolic compound, the sensor is based on the replacement reaction, through the color change we use the pyrocatechol violet and copper system can be sensitive efficient identification of oxalate ions in aqueous media. Detailed as follows:1. R1 is a based on the Rhodamine hydrazide new fluorescent probe with optical properties, it reaction with 5-chlorosalicylaldehyde in ethanol. The detection of the spectroscopy is in methanol and HEPES (v:v=1:1) buffer solution. Through a series of tests, we found that the new probe we measured can well recognition Cu2+ by UV-visible absorption spectra, the detection limit was 10-3M, the color of the solution is changed, it from colorless to dark red, In contrast, it can be well test VO2+ in the fluorescence spectrum, the detection limit was 10-7M. This is the first sensor that is capable of detecting Cu2+ and VO2 using two different modes.2. It assumed to involve the competition between the oxalate anion and PV molecular to coordinate with Cu2+, anion coordinated with the copper ions, releasing the free PV, PV will return to its original color and spectral properties. For this result, we take filter experiment for the anions, we found thatit can rapidly, sensitively, and quantitatively to detect the C2O42- in the solution.3.The vegetables contains a lot of C2O42-, we act fresh spinach as the experimental samples, considering PV-Cu2+ as the detection system, we detect the C2O42- quantitatively in the standard titration by this experiment whether we approach the practical value.