Development On The Sleep Improvement Functional Beverage Rice Wine

The sleep is one of the important physiological phenomenons in our daily life; it closely related to our physical and mental activity, it has an important effect on people's health and life. Good sleep will make people refreshed, so more and more people have drawn attention to the problem of sleeping quality and insomnia. However, with the rapid development of the modern society, the pace of people's life become more and more increasingly, the working pressure and others'become larger. As a common disease, Insomnia bothers us in daily life.Chinese herbal medicine is a nation's treasure and there is unique recognition on improve sleep and insomnia in traditional Chinese medicine. According to the clinical data observed by the Traditional Chinese Medicine, it treats mainly sleep-disorder somnipathy with Chinese medical prescriptions. Currently, more and more scholars are studying path mechanism of insomnia treatment by Chinese herbs. It attracts attention from the entire world for its effect in clinical practice that there is unique curative effect on improving sleep quality with the Traditional Chinese medicines. In this paper we study the development of enhancing sleep rice wine.According to the physical and chemical properties of jujuboside that is the effective element of traditional Chinese medicine and design extraction. According to the Box-Behnken of central composite experimental design principles, the response surface method with 3 factors and 3 levels was adopted. The results showed that the optimum conditions were that ethanol concentration of 69.8%, ultrasonic temperature of 44.6℃, ultrasonic power of 74.1w, Under such conditions the actual extraction yield of jujuboside A could be up to 321.09μg/g. This method can be used to extract jujuboside from semen ziziphi spinose.The jujuboside content was detected by HPLC, Mobile phase to use gradient elution: acetonitrile– water(20:80) at first 5min, acetonitrile concentration gradient up to 70% at 5~20 min, acetonitrile concentration gradient back to 20% at 20~22 min and detection at 203nm. Gradient elution can separate target material and other components.It was studied by measuring the spontaneous activity experimental of mice, the experiments of sleep-induced directly, prolonging sleeping time induced by pentobarbital sodium and affecting mouse sleeping rate under the threshold hypnogenesis dosage of pentobarbital sodium. The results show that the jujuboside possess calm and hypnotic effects.We select four kinds of medicinal and edible raw material, including red date, lily bulb, shelled cedar seed, honey to get water extract are added ultrasonic extract of jujuboside to compatibility experiment. And use high quality rice wine as the wine base, developed a low degree, nutrition, sleep function improvement rice wine beverage. Then we carry through the second pharmacology experiment to test the effect with the Jujuboside extract and water extract of red date, lily, and shelled cedar seed. Brought forwards a new medicine model and interpreted the mechanism of hypnogenesis.