Coenzyme Q₁₀ Extraction And Purification Methods Research

A simple method is established which use supercritical carbondioxide to extract coenzyme Q₁₀ from fermentative product and purify it through twice crystallization with acetone: soak the material with solvent A (0.3mL/g) for 2h, then extract with supercritical carbondioxide. The optimized extract conditions are as follow: extract temperature 30℃, extract pressure 22MPa, extract time 1.5h, CO₂ flow rate 4.0L/h, seperating temperature 50℃; acetone crystallization conditions for the extract are: acetone:extract=10:1(v/w) for the first time, acetone:raw=4:1(v/w) for the second time, dissolve the extract/raw in 50℃, vacuum filtrate with heat, then keep detem-perature rate in 1℃/2min, hold in 0℃for 4h. The total yield of Q₁₀ is about 90%, with crystall purity over 98%.Another feasible extract method for the fermentive product is reflux with petroleum ether. Its optimized conditions are as follow: petroleum ether(60-90℃): material=10:1(v/w), refluxing for 1h, with twice extract. The yield of Q₁₀ is 96.8%, with crystall purity 27.0%.12 different samples of tobacco leaves are assayed for the content of coenzyme Q₁₀, with Liaodong G70 (B₂F) containing the most. Q₁₀ content is high in leaves of Liaoning area , followed with Southeast area, Southern area and Central area, Jilin Yanshai 3^# takes the last position, upper leaves seem containing more Q₁₀ than medium.