Establishment And Application Of EST-SSR Markers Based On Faba Bean (Vicia Faba L.) Genome

Faba bean (Vicia faba L.) is one of the most important legumes in the world. It provides an important source of protein and starch for human diets in many countries.The main faba bean producers of the world in China and Europe. Due to its partial cross-pollination, faba bean is highly heterogeneous and consequently contains broad genetic variation within varieties and landraces, which make the conservation of germplasm resources more expensive and difficult. However, despite its long history of cultivation and economic importance, the number of molecular markers available for faba bean is still scarce. Therefore, developing more reliable and efficient molecular markers would be valuable in diversity analysis and resource conservation for faba bean.EST-derived microsatellite markers (also known as EST-SSR markers) have become a powerful tool in plant genetic analysis and breeding research. Due to their derivation from transcripts, these markers have some intrinsic benefits over genomic SSR markers: they are easier and less expensive to develop, possess higher transferability to related species, and are more closely connected with known function genes.5031 ESTs from Genbank database in NCBI, 1148 were obtained from 5031 Broad bean ESTs. We found 107 ESTs by software, accounting for 9.32% of ESTs were mined out. Trinucleotide repeats were the most abundant repeat class, and accounted for 44.86% of all found SSRs. And AAG are the most frequent motifs in trinucleotide, accounting for 12.5%.AG and TC are the most frequent motifs in dinucleotide, accounting for 33% each other. However,there were no significant differences among other types. In this research, we report for the first time the isolation and characterization of EST-SSRs in faba bean, including their potential for diversity analysis in different individuals and their transferability to a related pea species (Pisum sativum L.). A total of 11 novel EST-SSR loci were generated and characterized when tested on four populations of 29 faba bean individuals from China and Europe with two to three alleles per locus. The polymorphism information content value ranged from 0.06 to 0.43 with an average of 0.2919.Genetic similarity estimates from 11 SSRs showed two main cluster that comprised all Chinese genotypes and European genotypes, respectively. An average genetic similarity of 73.52% among the Chinese individuals. The results of this study proved that the EST-SSR marker is very effective in evaluation of faba bean germplasm.Furthermore, transferable analysis revealed that 72.73% amplified in Pisum sativum L., 75.00% detected polymorphism.These results indicate the potential of these newly developed markers for furt-her genetic investigations of pea.