An Established Genetic Map Based On Large And Small Grain Strain Of Brassica Napus

Gene mapping refers to the transfer of fang on a chromosome to determine the position of particular genes and gene MHD, according to the relative distance between the gene location data, each chromosome loci found on mapping are drawn. The establishment of genetic map need to have enough quantity of polymorphism, while the traditional mark genetic marker difficult to achieve ideal effect, greatly limits the establishment of genetic map. This study adopts SSR markers as a genetic linkage map construction, the molecular markers of microsatellite DNA markers on chromosomes are widely distributed, has the rich polymorphism, and low cost analysisThis research use the material by the Chinese academy of agricultural sciences oil-bearing crops biology key open lab, this material through continuous selfing and traits choice, has won the grain weight genes than pure big kernel strain and small grain of strain. On the basis of the materials, the author USES the gap of two kernel weight difference segregation, and really hybrid identification of hybrid, reoccupy identified a true hybrid selfing produce F2 group, this F2 group is the object of this studyThis study used for many generations the material into the pure department obtained from big kernel strain and small grains with them, strains parents get F2 population is this research object.Kernel weight gene polymorphism between parents first find the SSR markers with, then use these markers marker genes for F2 group examination and analysis data type.From published in Brassica napus 1. SSR primers for 551 picked out of primers, this 551 covered the entire genome, rapeseed using these primers detection polymorphism, mutual 107 primer detect polymorphism, specificity of detection ratio of 19.4%, the specific bands to 144. Choose from these primers on SSR primers from 48 to capacity for 190 plants of F2 group type analysis marker genes. Will get genotype data using Joinmap3.0 linkage analysis, about 25% on the mark shows significant partial separation, turn to small grain checklist of have five, accounted for parents to big grain,41.7% have 7, accounting for 18.3% and 58.3%. And we got five chain group, the integration of the 16 SSR markers, total cover length 295cM.The linkage map and the system of SSR molecular builded up make preparation for the subsequent experiment provides certain foundation.