Studies On Plant Regeneration, Callus Induction And Conservation Of Peach Rootstock GF677

Experiment was conducted with the leaves of peach rootstock GF677 (P.amygdalus×P.persica) in vitro for studying the effects of different basic medium, plant growth regulator types and content, carbon source content, dark period on adventitious shoot regeneration and callus induction, conversation. The main results were as follows:Firstly, The axillary germination percentage reached 90.90% late in June, and the growth condition was well. The proliferation culture medium for GF677 in vitro was LP+6-BA 0.75 mg/L+IBA 0.3 mg/L+GA3 0.5 mg/L+sucrose 30.0 g/L+agar 6.8 g/L. The plants were subcultured for every 28 d.Secondly, the optimal medium for rooting of GF677 in vitro was MS+IBA 1.0 mg/L+ NAA 0.1 mg/L+PG 120.0 mg/L+sucrose 30.0 g/L+agar 6.8 g/L. It produced the highest rooting percentage of 100% and a mean of 10.72 root number and of 3.72 cm length.Thirdly, the optimal medium for adventitious shoot regeneration from leaves of GF677 in vitro was LP+6-BA 4.0 mg/L+2,4-D 0.2 mg/L+AgNO3 0.5 mg/L+sucrose 20.0 g/L+ agar 6.0 g/L. The leaf explants wounded by transverse cuts triple vertically the midrib and incubated with adaxial side in contact with the medium and transferred to the light culture after 21 d dark treatment. The adventitious shoot regeneration rate was 13.33%.Fourthly, the optimal medium for adventitious shoot regeneration from the preconditioned leaves of GF677 in vitro was SH+6-BA 3.0 mg/L+NAA 0.3 mg/L+ AgNO3 0.5 mg/L+sucrose 20.0 g/L+agar 6.0 g/L. The leaves were transferred to light culture after 21 d dark treatment,with 9.93% of regeneration rate.Fifthly, the optimal medium for callus induction from the leaves of GF677 in vitro was LP+TDZ 1.0 mg/L+6-BA 0.6 mg/L+2,4-D 0.2 mg/L+AgNO3 0.5 mg/L+Sorbitol 20.0 g/L+agar 6.0 g/L. The young leaves along with their petioles from 4-week-old in vitro-grown shoots were used as explants, which were transferred to light culture after 21d dark treatment.At last, the optimal medium for callus conservation of GF677 was LP+6-BA 0.5 mg/L+ 2,4-D 0.5 mg/L+CH 0.2 g/L+sucrose 30.0 g/L+agar 6.0 g/L. The callus was in darkness all along and subcultured for every 21 d.