Vitrified Plantlets Recovery And Petiole Regeneration Of Peach Rootstock(Prunus Persica)

It is difficult for peach to regenerate adventitious buds from in vitro-cultured explants. This experiment mainly studied about vitrification recovery and petiole regeneration as well as the callus regeneration and preservation of the peach rootstock(Prunus persica), The following is the summary of results :1. To improve agar concentration from 6.8 g/L to 8.0 g/L of the subculture medium,which is the LP basic medium supplied with 6-BA 0.2 mg/L, IBA 3.0 mg/L, Ad 3.0 mg/L and sucrose 30 g/L, we can get the maximum recovery rate 68.22% of vitrified plantlets.The recovery and normal plantlets had no significant difference in leaf water content,tissue structure and cell morphology and later following the generation of the vitrification rate, indicating that the recovered seedlings has reached the normal level.2. The medium with the highest petiole regeneration rate is WPM + KT0.5 mg/L +IAA0.5 mg/L+Ad3.0 mg/L, and the following are MS+KT1.5 mg/L+IAA1.0 mg/L +Ad3.0 mg/L and SH+KT 1.5 mg/L+ IAA 0.5 mg/L+ Ad 3.0 mg/L,the regeneration rate are as follows: 14.49%,11.59% and 10.15%, but there is no significant difference between the three treatments.The p H of optimal regeneration medium is 5.8; When adding 10 g/L sorbitol and sucrose 20 g/L to the subculture medium, the petiole regeneration rate was7.14%, other concentration of petiole was no regeneration; when CPPU concentration was0.5 mg/L, NAA concentration from 0.05 to 0.1 mg/L, petiole regeneration rate increased to 4.35%; callus texture of petiole explants is better than that of ordinary petiole regeneration, but did not have a adventitious bud.3. On callus proliferation and regeneration test, less the amount of stem base,the browning of callus proliferation is more serious; peach callus cultured by LP +6-BA 1.0mg/L+NAA 0.5 mg/L+ sucrose 30 g/L+ agar 6.8 g/L achieved rapid proliferation; adding PEG and glycerol as a carbon source to the media did’t regenerate adventitious buds,while LP +CPPU0.1 mg/L+NAA 0.5 mg/L+CH 0.4 g/L+15 or 20 ml/L glycerol and agar6.8 g/L can be considerd for light culture callus proliferation; LP +TDZ3.0 mg/L+ZT0.5mg/L+sucrose30 g/L+agar 6.8 g/L for dark cultured callus; When cultured with LP+CPPU 0.1 mg/L+NAA0.5 mg/L + agar6.8 g/L+ sucrose15 g/L+ sorbitol15 g/L of stems thin slices of peach for callus induction, callus had good texture and color.