The Preliminary Rearsch On Technique System Of Tissue Culture And Rapid Propagation For Bambusa Ventricosa McClure

The use of biotechnology for genetic improvement of bamboo is one of the researches of bamboo breeding. And the basic research of bamboo genetic improvement breeding is to build a stable bamboo regeneration system. Bambusa ventricosa McClure is a graceful bamboo for view. This research focused on the buds proliferation and plant regeneration, callus culture. The results are as follows:1. The buds proliferation and plant regeneration of Bambusa ventricosa McClure(1) The best conditions for stalk bud germinating was:explant soaked by 70% alcohol 30s, used 0.1% HgCl2, disinfected 5minutes, MS basic medium,6-BA 5mg/L.(2) The suitable medium for buds induction was MS+6-BA 6mg/L+NAA 0.2mg/L+sucrose 30g/L+agar 6.5g/L.(3) The fessible medium for buds proliferation was MS+6-BA 5mg/L+TDZ 0.05mg/L +NAA 0.2mg/L+sucrose 30g/L+agar 6.5g/L.(4) The optimizing medium for regenerated plantlet rooting was MS+NAA 0.5mg/L+IBA 0.1mg/L+6-BA lmg/L+sucrose 30g/L+agar 6.5g/L.(5) The regenerated Plantlet transplanting was perlite:vermiculite:loam(1:1:1). 2. Callus culture of Bambusa ventricosa McClure(1) The best preparation conditions for callus culture was sucrose 30g/L, Vc100mg/L, sterile stem segments as explants is the most appropriate choice.(2) The fessible medium for callus induction was MS+2,4-D 4mg/L+6-BA 0.5mg/L+sucrose 30g/L+agar 6.5g/L.TypeⅠ:Pale white to light yellow, compact structure, granular, hard texture of the embryogenic callus which can be used to further proliferation and differentiation of experimental.TypeⅡ:light brown, water damaged seriously, scattered in the tight or loose friable callus, and such kind of callus is vulnerable to browning.TypeⅢ:White or translucent, loose, friable non-embryogenic callus.(3) The suitable medium for callus proliferation was:MS+2,4-D 3mg/L+NAA 0.5mg/L +6-BA 0.1mg/L+sucrose 30g/L+agar 6.5g/L.