Separation, Identification And Drug Susceptibility Test Of Fowl Typhoid Salmonella

Fowl Typhoid is caused by salmonella typhimurium,with the characteristic of liver, spleen and other essential organs lesions and diarrhoea,which is a septic infectious disease that occurs in adult chicken and young chicken. The disease can be transmited horizontally, also can be transmited vertically.It is a great damage to chicken industry.In recent years, antibiotics have been excessively used or abused to treat and prevent this disease. Under the pressure of antibiotics selectivity, drug-resistance of Salmonella has been significantly enhanced accompanying with continuously widened spectrum of multiple drug resistance, which has caused great difficulties on prevention and treatment of the disease.In this study,we do research on the infection of salmonella and drug resistance of pathogenesis chicken in order to provide certain theoretical basis and the reference data for using drug rationally in clinical.1. 60 specimens of liver and lineal of pathogenesis chickens were separated from a chicken house in Fuping a county of Shaanxi province . Under aseptic conditions,these specimens were stroken inoculation on ordinary agar plate and incubated at 37°C for 24 h. After Gram's method,we can see a large number of both ends of the rounded, short, and most of the existence of a single gram-negative small bacilli under the microscope. 13 representative strains were used for biochemical tests The result showed that the 13 strains isolated from chickens are in line with the biochemical characteristics of Salmonella typhi. Pathogenicity test showed that all the isolates have serious Pathogenicity except S2 and S11.2. 13 representative strains were used for antimicrobial susceptibility test with 21 kinds of antibiotic in disc diffusion method.The result showed that isolates were highly sensitive to Neomycin and Amikacin, were moderately sensitive to furazolidone and kanamycin and were resistance to Cotrimoxazole, Tetracycline, Pioneer and so on.3. So Amikacin had been use by intramuscular injection and Neomycin had been used with water to treat Fowl Typhoid for the following pathogenesis chickens and Furazolidon had been used with feed.The result was Significant. After 5 days, pathogenesis chickens restored appetite and their Spirit improved greatly. Yellow-green feces also reduced significantly. The incidence rate decreased greatly with decreasing from 50% to 5%.After another 1 week , the incidence rate decreased to 0.