The Influence Of Different Protein Sources On Rumen Environment And MCP Content And Microbial Population Structure On The Northern Shannxi Villus Goat.

The rumen fermentation were different by feed Different diets, and the rumen digestion of different diet were not the same too.Balance of the rumen environment was affected by various factors, such as the type and amount of feed, the feeding time, the flow rate of rumen contents and rumen microbial population number and structure etal. This experiment was to study the influence of different protein Sources on rumen environment and the MCP content and microbial population structure on the Northern Shannxi Villus Goat. Using four similar body size Northern Shannxi Villus Goats with Permanent rumen fistula, designed by 4×4 Latin square.With four protein diets: cottonseed treatment (A group), corn gluten treatment (B group), soybean treatment (C group) and rapeseed treatment (D group), to study the influence on rumen PH, ammonia nitrogen concentration, rates of feed protein degradability in the rumen, rumen MCP concentration and microbial structure. The study consist two parts, the first part was the influnce on rumen environment by feeded with different protein sources to Northern Shannxi Villus Goats and the protein digestibility in the rumen. The second part was the influnce of different protein sources to the content of rumen microbial population and the population structure. From these two aspects, we could economy and reasonable add different protein feeds to Northern Shannxi Villus Goats, to efficiency use of protein feed ,reduce feeding costs and Increase economic benefits efficiency. At the same time to provide the theoretical basis for the protein nutrition on ruminants and for rational use feed resources.The result indicated that the pH value were distributed between 6.07～6.61, each group reached the lowest point at 2h,the soybean meal was 6.07,Compared with the other three groups, the difference was significant (p<0.01), other times the groups was not significant (p> 0.05). NH₃-N concentration range was 9.91～26.56mg/100mL, look at the average of each group, A group and D group showed no significant difference (p>0.05), among other groups at different time showed significant differences (p<0.05) or highly significant (p<0.01). CP digestibility of four protein feeds were significant, the highest digestibility was soybean meal. MCP concentration in the rumen after feeding, as the time gone by, decreased first, and then increased,then decreased. The difference between the groups before intake was not significant. C group compared with the other three groups, maintaining a high level, with each group significantly different (p<0.05 or p<0.1).Different protein diets on Northern Shannxi Villus Goat rumen protozoa have a significant impact. Average concentrations of the 4 protozoa protein were 14.31, 14.26, 15.0, 14.38 mg/100mL, where C group was significantly (p<0.01) higher than the other three groups. Changes of bacteria were Similar with protozoa. Overall, as the time gone by,the rumen protozoa and bacteria's concentration after feeding, showed decreased first, then increased and then decreased. Compare the ratio of protozoa and bacteria proteins found that within 4 hours after feeding was no significant difference between groups, at 6h after feeding, B group and D group showed significant difference (p<0.05), while the C group compared with other groups were significant difference at the 8～10h (p<0.01). See from the averagement of each group, the difference was not significant (p>0.05) between the groups.In summary, the rumen degradability of different protein feeds was different, the influce of different protein diets on Northern Shannxi Villus Goat's rumen environment, rumen microbial protein, microbial community structure was different.