In Vitro Effects And Biochemical Characteristics From Material Of Juvenile And Mature Originin Quercus Variabilis

Quercus variabilis is a Quercus tree of Fagaceae, which is the one of widely distributed arbors, and played a huge role in local economic development and ecological balance of protection. Q. variabilis is propagated mainly by seeds. Severe invasion by weevil,difficult to storage seeds over a long period of time, difficult to cutting,as well as offspring prone to genetic differentiation, have bad effects on the promotion of using the good genotypes. Modern tissue cultural techniques have a new way for the trees of rapid nutrition organ propagation, n and have been widely used in production. However, due to physiological aging, it is more difficult for adult materials to plant regeneration through organogenesis. Therefore, studies on the cyclophysis of Q. variabilis in vitro effect of are great significance. In this paper, juvenile and mature stem segments are as explants, which were compared on the abilities of proliferation and rooting in vitro, Moreover we studied the relationship between the changes of antioxidant enzyme activity and the ability of proliferation, also the ability of rooting. The results are as follows.1. The ability of elongation and rooting from identical sources of nodal segments from different clones are similar, the antioxidant enzyme activities were not significantly different after the each cultural stage.2. The ability of elongation from juvenile is strongest, the proliferation ratio is 2.11, and the axillary shoots number, length of tallest shoot and green leaves number from juvenile nodal segment were more than mature, which were significant (P<0.05) after the last elongation.3. The ability of rooting from juvenile nodal segment is strongest, rooting rate that is 83.5% and roots number per explant that is up to 3, which were significant (P<0.05), were more than mature.4. 3 sources of nodal segment in antioxidant enzyme activities were more than those of another elongation stages after the last elongation and rooting, which were all significant (P<0.05), the highest value was that SOD is 74.53U/mg and CAT is 53.38U/mg after rooting.5. IBA and NAA promote rooting with different concentration, the antioxidant enzyme activity will increase as the concentration of high-born; the antioxidant enzyme activities are highest with mature at all hormone concentration.6. Different combined hormones for explants on rooting, the highest antioxidant enzyme activity is mature with IBA1.0mg / L, NAA0.2mg / L; IBA for antioxidant enzyme activities is significant (P<0.05).