Effects Of Broussonetia Papyrifera Leaf On Lipid Metabolism Of Nutritive Obese Mice

As a kind of deciduous tree, Broussonetia papyrifera widely distributed in China, it contains many kinds of biological active component, such as polysaccharides, alkaloids, flavonoids, glycosides, etc. Broussonetia papyrifera have muti-pharmacological actions, such as antivirus, anti-oxygenation and antibacterial, received more and more attention in recent years. The present work was focused on the effects of Broussonetia papyrifera leaf on lipid metabolism of the nutritive obese mice induced by high fat diet, and to provide a scientific basis for the further development of Broussonetia papyrifera resources.Experiment 1. 150 healthy Kunming mice (aged 7-8weeks) were randomly divided into 2 groups, the control group had 30 animals with 3 repetitions, fed basal diet, and the experiment group had 120 mice with 12 repetitions, fed high fat diet. The body weight and lee's index were determined to evaluate the nutritive obese effect induced by high fat diet .The result indicated that the body weight of the mice, fed high fat diet for 7 weeks, was significantly increased by 17.14%(p<0.01), meanwhile the lee's index was significantly increased by 6.53%(p<0.01).Experiment 2. 118 nutritive obese mice were randomly divided into 3 groups, each had 36 animals with 3 repetitions. Mice in control group were fed high fat diet, animals in experiment group 1 and 2 were respectively fed high fat diet supplemented 2% and 5% Broussonetia papyrifera leaf. The serum content of GLU, TG, TC, FFA,HDL LDL and parameters SOD,GSH-PX and MDA associated anti-oxygenation of the mice were detected at 30,50 and 70 days during the experimental period. All of the animals were anatomized and the heart, liver, spleen and kidney were collected to determine the organ indexes, and the liver, spleen and kidney were also used to prepare tissue slices. The results were as follows:(1)The serum GLU, TG and FFA concentration of the obese mice in group 1 and 2, fed 2% and 5% Broussonetia papyrifera leaf, were significantly decreased than that of control group, the serum TC content were also decreased by 7.43% and 13.45% respectively, which implied that Broussonetia papyrifera leaf could promote energy conversion and consumption of the obese mice.(2)The serum HDL content of the obese mice in group 1 and 2 supplemented Broussonetia papyrifera leaf were significantly increased than that of control group, the serum LDL also slightly reduced, but there were no significant differences was observed. It meant that Broussonetia papyrifera leaf could promote TC transportation process from extra-hepatic tissue to liver, which was re-utilized as intermediate metabolites.(3)The body weight of the obese mice fed 2% or 5% Broussonetia papyrifera leaf were slightly decreased than that of control group (pï¹¥0.05).(4)The serum MDA content of the mice fed 2% and 5% Broussonetia papyrifera leaf were significantly lower than that of control group(P<0.01), no remarkable changes of serum SOD and GSH-PX activity were observed. The result here implied that 2-5% Broussonetia papyrifera leaf supplementation in the nutritive obese mice diet play an important role in elimination of peroxides, maintenance of antioxidant equilibrium status and holding SOD and GSH-PX activity to prevent lipid peroxidation.(5)There were no significant effects on the organ indexes of kidney, heart and spleen(p>0.05), but the liver indexes were significantly increased. It meant that Broussonetia papyrifera leaf supplementation in the diet had beneficial effects on lipid metabolism of the obese mice, including, relieving of fatty degeneration and improving fat metabolism of liver to prevent hepatic adipose infiltration from high fat diet induction.