Studies On Extraction Technology Of Key Components And Accumulation From Sophora Tonkinensis

Sophora tonkinensis, is a traditional Chinese medicine. it is record under pharmacopoeia of P.R.C. (2010 version). It has the ability for detoxification swelling relieving swelling and throat care.The project based on the study of ultrasound extraction processes, which was studying on wield S. tonkinensis's key components of total flavonoids, saponin, polysaccharide to do research on total flavonoids, saponin and polysaccharide, that got from different parts of S. tonkinensis and came from different places. The purpose of the project was to defined medicinal positions of S. tonkinensis. It could provide theoretical based for the utilize germplasm resources of S. tonkinensis and S. tonkinensis var. polyphylla scientifically and effectively. Meanwhile, compared the accumulation rules of the content of total flavonoids, saponin and polysaccharide from two botanical origin of S. tonkinensis and S. tonkinensis var. polyphylla.The research found out the optimum extraction process to extract total flavonoids of S. tonkinensis, extraction time 90 minutes, material-liquid ratio 1:30, extraction temperature 70℃, ethanol density 80%. Yield of total flavonoids was 1.312%. Optimum process for saponin of S. tonkinensis, extraction time 150 minutes, material-liquid ratio 1:30, extraction temperature 50℃, methanol density 90%. Yield of saponin was 3.890%. Optimum process for polysaccharide of S. tonkinensis, extraction temperature 70℃; material-liquid ratio 1:40, extraction time 150minutes. Yield of polysaccharide was 21.404%. It provided reference for industrial production, and could be a base of advanced research of S. tonkinensi's key components.The study showed, S. tonkinensis contains key components of total flavonoids, saponin, polysaccharide, the active component of S. tonkinensis was accumulated in the root. S. tonkinensis from Jinchai of Du'an had the highest content of total fiavonoids, Kuixu of Jingxi had the highest content of saponin and the Ludong of Jingxi had the highest content of polysaccharide.The result of the research on S. tonkinensis which were harvested in different month indicated,S. tonkinensis wield herbal products could be harvested during the whole year. S. tonkinensis and S. tonkinensis var. polyphylla's total flavonoids and saponin had little difference, while polysaccharide had an apparently difference. The result of the project provides evidents of that S. tonkinensis and S. tonkinensis var. polyphylla could be used as equivalent herbal-products.