Studies On The Propagation Of Brassica Pekinenis×B.oleracea

Research methods of increasing the Brassica pekinenis×B.oleracea (n=19) setting percentage and breeding multiples were study, including using saline and boricfertilizer processing the stigma during flowering phase, axillary cuttings before B. pekinenis×B.oleracea blossoming, in vitro culture of ovary and part stem section. The main results indicated as follows:1. The rate that the acetic carmine dyed B. pekinenis×B.oleracea pollen reached 71.5%, so this proved the B. peMnenis×xB.oleracea pollen was viable. In vitro culture with B. pekinenis×B.oleracea pollen, pollen germination rate was only 10.1%. Under the same conditions, the cabbage pollen germination rate was 76.7%. In different saline concentrations treatment on B. pekinenis xB.oleracea stigmas, the effect of 3% was best that sillqua percentage reached 18.39%, pollination number was 298 and 23 seeds were harvested, while in different boricfertilizer concentrations treatment the effect of 0.5% was best that sillqua percentage reached 13.05%, pollination number was 301 and 48 seeds were harvested.2. Different growth regulators processing axillary bud can enonnously increase the survival rate of cuttage. The treatment of IAA and ABT was better than that of NAA,when IAA concentration was 3mg/L, the average amount and length of root, rooting rate was respectively 21.9,7.4cm and 84.4%. The average amount and length of root, rooting rate was respectively 23.1,6.7cm and 86.7%,when ABT was 3mg/L.3.The effect of 0.1% HgCl2 disinfecting stems of B. pekinenis×B.oleracea was better than that of 10% NaClO.The effect was best that using 75% ethanol wiped explant surface of B. pekinenis×B.oleracea stems, then 0.1% HgCl2 disinfected for 12 min. Induction effect of composite plant growth regulator was superior to single plant growth regulator. Appropriate medium of inducing callus was MS+2,4-D 2.0mg/L+6-BA 0.3mg/L,the callus formation rates reached 85.83%. 4. In ovary culture, the optimum medium was MS+6-BA 1.0mg/L+NAA 0.2mg/L, in this treatment ovary expansion ratio reached 58.9% and got 2 seeds. The ovaries developing for 16 days after pollination were the best culture materials, and ovary expansion ratio reached 67.8%. Take the ovary without node and ovary expansion ratio reached 51.1%. When inoculated number was 90, Sseeds were gained.